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Capture and Release of Viable Circulating Tumor Cells from Blood

DOI :

10.3791/54435-v

October 28th, 2016

October 28th, 2016

8,182 Views

1Department of Pathology, University of Miami, 2SRI International, 3Department of Biomedical Engineering, DJTMF Biomedical Nanotechnology Institute at the University of Miami, University of Miami

A protocol to utilize a poly(N-iso-propylacrylamide) (PIPAAm) coated microfilter for effective capture and thermoresponsive release of viable circulating tumor cells (CTC) is presented. This method allows capture of CTC from patients' blood and subsequent release of viable CTC for downstream off-chip culture, analyses and characterization.

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