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Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts

DOI :

10.3791/57011-v

7:18 min

June 13th, 2019

June 13th, 2019

6,509 Views

1Department of Pathology, Icahn School of Medicine at Mount Sinai

We describe a detailed protocol for the isolation of tumor-initiating cells from human sarcoma patient-derived xenografts by fluorescence-activated cell sorting, using human leukocyte antigen-1 (HLA-1) as a negative marker, and for the further validation and characterization of these HLA-1-negative tumor-initiating cells.

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Isolation

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