Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes

8.0K Views

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08:52 min

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July 26th, 2019

DOI :

10.3791/59973-v

July 26th, 2019

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Saahir Khan¹, Aarti Jain², Omid Taghavian², Rie Nakajima², Algis Jasinskas², Medalyn Supnet², Jiin Felgner², Jenny Davies², Rafael Ramiro de Assis², Sharon Jan², Joshua Obiero², Erwin Strahsburger², Egest J. Pone², Li Liang², D. Huw Davies², Philip L. Felgner²

¹Division of Infectious Diseases, Department of Medicine, University of California Irvine Health, ²Vaccine Research and Development Center, Department of Physiology, University of California Irvine

Summary

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Influenza Antigen Microarray

Serum Antibodies

Influenza Strains

Antigenic Landscape

High throughput Measurement

Microarray Technique

Infectious Disease

Antibody Characterization

Antigen Targets

Microarray Printer

Nitrocellulose Slides

Chapters in this video

0:04

Title

1:27

Antigen Microarray Printing

3:01

Probe Sera Microarray Incubation

4:25

Quantum-Dot-Conjugated Secondary Antibody Labeling

5:16

Antibody Binding Capture

6:03

Antibody Binding Quantification

7:00

Results: Representative Influenza Antigen Microarray Analysis

8:03

Conclusion

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