September 18th, 2019
•We describe an in vivo method to investigate the functional loss of a specific gene in satellite cells by using a combination of cardiotoxin mediated injury of skeletal muscle and the injection of a self-delivering siRNA.
Related Videos
siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts
RNA Interference-based Investigation of the Function of Heat Shock Protein 27 during Corneal Epithelial Wound Healing
Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions
High Fat Diet Feeding and High Throughput Triacylglyceride Assay in Drosophila Melanogaster
Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection
CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy
Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System
Using Mouse Oocytes to Assess Human Gene Function During Meiosis I
Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved