JoVE Logo
Faculty Resource Center

Sign In

Isolation and Culture of Myofibers and their Satellite Cells

DOI :

10.3791/50074-v

11:57 min

March 22nd, 2013

March 22nd, 2013

60,107 Views

1Sprott Center for Stem Cell Research, Ottawa Hospital Research Institute, 2Department of Cellular and Molecular Medicine, University of Ottawa

Isolation and culture of myofibers is the gold standard in vitro system to study the transition of satellite cells through quiescence, activation and differentiation. Importantly, the single myofiber culture system preserves the myofiber/stem cell association, which is an essential component of the muscle stem cell niche.

Tags

Isolation

-- Views

Related Videos

article

Ex Vivo Assessment of Contractility, Fatigability and Alternans in Isolated Skeletal Muscles

article

Isolation and Culture of Neonatal Mouse Cardiomyocytes

article

Naïve Adult Stem Cells Isolation from Primary Human Fibroblast Cultures

article

Analysis of Embryonic and Larval Zebrafish Skeletal Myofibers from Dissociated Preparations

article

Isolation, Culture, and Functional Characterization of Adult Mouse Cardiomyoctyes

article

Using an Automated 3D-tracking System to Record Individual and Shoals of Adult Zebrafish

article

Assessment of Calcium Sparks in Intact Skeletal Muscle Fibers

article

Isolation and Characterization of Exosomes from Skeletal Muscle Fibroblasts

article

Isolation and Culture of Primary Synovial Macrophages and Fibroblasts from Murine Arthritis Tissue

article

Author Spotlight: Ex Vivo Protocol for Culturing Quiescent Muscle Stem Cells with Niche Components

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved