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Isolation and Culture of Myofibers and their Satellite Cells

DOI :

10.3791/50074-v

11:57 min

March 22nd, 2013

March 22nd, 2013

59,647 Views

1Sprott Center for Stem Cell Research, Ottawa Hospital Research Institute, 2Department of Cellular and Molecular Medicine, University of Ottawa

Isolation and culture of myofibers is the gold standard in vitro system to study the transition of satellite cells through quiescence, activation and differentiation. Importantly, the single myofiber culture system preserves the myofiber/stem cell association, which is an essential component of the muscle stem cell niche.

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