JoVE Logo
Faculty Resource Center

Sign In

Implantation and Monitoring by PET/CT of an Orthotopic Model of Human Pleural Mesothelioma in Athymic Mice

DOI :

10.3791/60272-v

December 21st, 2019

December 21st, 2019

6,497 Views

1MicroPET/SPECT/CT Imaging Laboratory, Centre for BioMedical Imaging (CIBM), Division of Nuclear Medicine and Molecular Imaging, University Hospitals and University of Geneva, 2Division of Thoracic and Endocrine Surgery, University Hospitals and University of Geneva

This article describes the generation of an orthotopic mouse model of human pleural mesothelioma by implantation of H2052/484 mesothelioma cells into the pleural cavity of immunocompromised athymic mice. The longitudinal monitoring of the development of intrapleural tumors was assessed by non-invasive multimodal [18F]-2-fluoro-2-deoxy-D-glucose positron emission tomography and computed tomography imaging.

-- Views

Related Videos

article

Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro

article

Determining Optimal Cytotoxic Activity of Human Her2neu Specific CD8 T cells by Comparing the Cr51 Release Assay to the xCELLigence System

article

Induction of Graft-versus-host Disease and In Vivo T Cell Monitoring Using an MHC-matched Murine Model

article

Isolation of Human Umbilical Vein Endothelial Cells and Their Use in the Study of Neutrophil Transmigration Under Flow Conditions

article

Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation

article

piggyBac Transposon System Modification of Primary Human T Cells

article

Right Ventricular Systolic Pressure Measurements in Combination with Harvest of Lung and Immune Tissue Samples in Mice

article

A Simple and Efficient Method to Detect Nuclear Factor Activation in Human Neutrophils by Flow Cytometry

article

4D Multimodality Imaging of Citrobacter rodentium Infections in Mice

article

Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved