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An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization

DOI :

10.3791/50332-v

June 12th, 2013

June 12th, 2013

18,386 Views

1Department of Cardiology, University of Heidelberg

Monocyte-derived macrophages are important cells of the innate immune system. Here, we describe an easy to use in vitro model to generate these cells. Using gradient centrifugation, negative bead isolation and specific cell culture conditions, monocyte-derived macrophages can be generated for phenotypic and functional studies.

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In Vitro Model

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