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Opsono-Adherence Assay to Evaluate Functional Antibodies in Vaccine Development Against Bacillus anthracis and Other Encapsulated Pathogens

3.3K Views

13:47 min

May 19th, 2020

DOI :

10.3791/60873-v

May 19th, 2020


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Opsono Adherence Assay

Chapters in this video

0:04

Introduction

1:21

Plating of the Cell Line, RAW 264.7 and Bacterial Culture

6:29

FITC-labeling of Bacteria and Bacterial Opsonization

8:46

Adherence Assay and Fluorescent Labeling of Mammalian Cells

10:22

Microscopy

11:33

Results: Representative Micrographs Collected During an OAA Experiment to Evaluate the Efficacy of an Anthrax Vaccine

13:01

Conclusion

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