With TRAP, we can investigate developmental processing at cellular resolution. We use cell-type-specific promoters to drive a tagged ribosomal sub-unit, and from there we can isolate polysome RNA. This protocol yields high quality RNA, but in very
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Translating ribosome affinity purification (TRAP) offers the possibility to dissect developmental programs with minimal processing of organs and tissues. The protocol yields high-quality RNA from cells targeted with a green fluorescent protein (GFP)-labeled ribosomal subunit. Downstream analysis tools, such as qRT-PCR or RNA-seq, reveal tissue and cell type-specific expression profiles.