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In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana

DOI :

10.3791/55975-v

September 11th, 2017

September 11th, 2017

10,602 Views

1Division of Biological Science, Graduate School of Science, Nagoya University, 2Higashiyama Live-Holonics Project, JST-ERATO, Nagoya University, 3Institute of Transformative Bio-Molecules (ITbM), Nagoya University

This manuscript describes an in vitro ovule cultivation method that enables live-cell imaging of Arabidopsis zygotes and embryos. This method is utilized to visualize the intracellular dynamics during zygote polarization and the cell fate specification in developing embryos.

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In Vitro Ovule Cultivation

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