JoVE Logo
Faculty Resource Center

Sign In

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

DOI :

10.3791/62852-v

14:42 min

September 23rd, 2021

September 23rd, 2021

3,339 Views

1Graduate School of Genome Science & Technology, University of Tennessee Knoxville, 2Bredesen Center for Interdisciplinary Research, University of Tennessee Knoxville, 3Biosciences Division, Oak Ridge National Laboratory

The protocols describe high-performance liquid chromatography methods coupled to refractive index or mass spectrometric detection for studying metabolic reactions in complex lysate-based cell-free systems.

Tags

Liquid Chromatography

-- Views

Related Videos

article

Alginate Microcapsule as a 3D Platform for Propagation and Differentiation of Human Embryonic Stem Cells (hESC) to Different Lineages

article

Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies

article

Quantitative Characterization of Liquid Photosensitive Bioink Properties for Continuous Digital Light Processing Based Printing

article

Soybean Hairy Root Transformation for the Analysis of Gene Function

article

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

article

Author Spotlight: Advances in Evaluating Human Lung Epithelial Cells' Response to Metal-Organic Frameworks

article

Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate

article

Methods for Electroporation and Transformation Confirmation in Limosilactobacillus reuteri DSM20016

article

Author Spotlight: Understanding Chronic Lung Diseases Using 3D Printed Phototunable Hydrogels

article

Author Spotlight: Advancing Coral Culture — Creating a Semi-Quantitatively Controlled Microenvironment System to Counter Current Limitations

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved