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JoVE Journal

Behavior

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High-Throughput Method for Measuring Alcohol Sedation Time of Individual Drosophila melanogaster

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06:15 min

April 20th, 2020

April 20th, 2020

6,899 Views

0:04

Introduction

0:57

Construction of the Testing Apparatus and Filming Chamber

2:11

Preparation of the Testing Apparatus and Flies

3:32

Testing the Flies

4:49

Results: Alcohol Sedation Times of DGRP Lines RAL_177 and RAL_555

5:37

Conclusion

Current methods to measure alcohol sensitivity in Drosophila are designed to test groups of flies. We present a simple, low-cost, high-throughput assay for assessing alcohol sedation sensitivity in large numbers of single flies. The method does not require specialized tools and can be performed in any laboratory using common materials.

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