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Isolating Central Nervous System Tissues and Associated Meninges for the Downstream Analysis of Immune cells

DOI :

10.3791/61166-v

May 19th, 2020

May 19th, 2020

7,801 Views

1Department of Neurology, Geisel School of Medicine, Dartmouth-Hitchcock Medical Center, 2Program in Experimental and Molecular Medicine, Dartmouth College

This paper presents two optimized protocols for examining resident and peripherally derived immune cells within the central nervous system, including the brain, spinal cord, and meninges. Each of these protocols helps to ascertain the function and composition of the cells occupying these compartments under steady state and inflammatory conditions.

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Isolating Central Nervous System Tissues

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