Name: isolating central nervous system tissues and associated meninges for the downstream analysis of immune cells
Uploaded: 2020-05-19T14:45:00.000+00:00
Duration: 9 min 35 s
Description: Watch this Scientific Journal Video about Isolating Central Nervous System Tissues and Associated Meninges for the Downstream Analysis of Immune cells at JoVE.com

The authors thank the staff of the Center for Comparative Medicine and Research (CCMR) at Dartmouth for their expert care of the mice used for these studies. The Bornstein Research Fund funded this research.

The authors have nothing to disclose.

Methods for evaluating the cellular composition in the CNS compartment during homeostasis and disease are essential for understanding the physiological and pathological states of the CNS. However, despite serving as an important barrier in the CNS and housing a diverse array of immune cells, the meninges are often omitted from analysis because many conventional tissue extraction methods for the brain and spinal cord do not allow for the collection of these membranes. This omission is a critical limitation in the advancem...

The central nervous system (CNS) is an immunologically specialized site. The CNS parenchyma, excluding the CSF space, the meninges, and the vasculature, is classically viewed as an immune-privileged site1,2,3,4,5 and is relatively devoid of immune cells during homeostatic conditions2,6,7. In contrast, the meninges, comprised of the dura, arachnoid, and pia layers, are crucial components of the CNS compartment, actively participating in homeostatic immune surveillance and inflammatory processes during disease pathogenesis3,6,7,8. During steady state conditions, the meninges support numerous immune sentinel cells, including innate lymphoid cells (ILC), macrophages, dendritic cells (DC), mast cells, T cells, and to a lesser extent, B cells9,10,11.
The meninges are highly vascularized structures and contain lymphatic vessels that provide a lymphatic connection between the CNS and its periphery8,12,13,14. In inflammatory conditions induced by CNS injury, infections, autoimmunity, or even neurodegeneration, peripherally derived immune cells infiltrate the parenchyma and alter the immune landscape within the meninges. Following cell infiltration, the meninges may represent a functional niche for peripherally derived immune cells, promoting immune cell aggregation, local immune cell activation, and long-term survival in the CNS compartment. Prominent meningeal inflammation is observed in multiple diseases affecting the CNS, including multiple sclerosis (MS)15,16,17,18,19, stroke20,21, sterile injury22,23 (i.e., spinal cord injury and traumatic brain injury), migraines24, and microbial infection25,26,27,28,29. Thus, the characterization of resident cells and peripherally derived immune cells in the meningeal compartment is essential for understanding the role of these cells during steady state conditions and disease pathogenesis.
The extraction of the brain, spinal cord, and meninges from the cranium and vertebral bodies is technically challenging and time-consuming. There are currently no techniques available for the rapid extraction of the brain with all three meningeal layers intact. While laminectomy yields excellent spinal cord tissue morphology and preserves the meningeal layers, it is both extremely time-consuming and complicated30,31. Conversely, more conventional extraction methods such as the removal of the brain from the cranium and the hydraulic extrusion of the spinal cord facilitate the quick extraction of the CNS tissue, but both the arachnoid and dural meninges are lost with these techniques30,31. The omission of dura and arachnoid layers during conventional isolation of brain and spinal cord tissues results in an incomplete analysis of the cells within the CNS compartment. Thus, the identification of new techniques focused on the quick extraction of CNS tissues with intact meninges is crucial for the optimal analysis of the CNS compartment.
This manuscript presents two methods for the rapid extraction of the brain, spinal cord, and meninges from mice, facilitating the downstream analysis of resident cells and peripherally derived immune cells in the CNS parenchyma and meninges. These optimized protocols focus on 1) isolating single-cell suspensions for downstream analysis and 2) preparing tissue for histological processing. Obtaining single-cell suspensions from the brain, spinal cord tissue, and dural and arachnoid meninges32 allows for the simultaneous analysis of cells residing in both the parenchymal and meningeal compartments. Single-cell suspensions can be used in different applications, including cell culture assays to perform in vitro stimulation33, enzyme-linked immunospot (ELISpot)28,34,35, flow cytometry36,33, and single-cell37 or bulk transcriptomics. Additionally, the optimized protocol for decalcification of whole brains and spinal cords with intact skulls or vertebral columns, respectively, allows for the gentle decalcification of the surrounding bone, leaving the meninges intact and preserving the tissue morphology. This method allows for the selective identification of proteins or RNA using immunohistochemistry (IHC) or in situ hybridization (ISH) techniques within both the parenchymal and meningeal spaces. The characterization of the phenotype, activation state, and localization of resident cells and peripherally derived immune cells within the CNS may provide information essential to understanding how individual cell types in the CNS compartment contribute to homeostasis and disease pathogenesis.

<table><tbody><tr><td>Aluminum foil</td><td>any</td><td>N/A</td><td></td></tr><tr><td>Bovine Serum Albumin</td><td>ThermoFisher Scientific</td><td>37002D</td><td></td></tr><tr><td>Centrifuge</td><td>Beckman Coulter</td><td>Allegra X-12R centrifuge</td><td></td></tr><tr><td>Collagenase I</td><td>Worthington</td><td>LS004196</td><td></td></tr><tr><td>Conical tube, 15 mL</td><td>VWR</td><td>525-1069</td><td></td></tr><tr><td>Conical tube, 50 mL</td><td>VWR</td><td>89039-658</td><td></td></tr><tr><td>Cover glass</td><td>Hauser Scientific</td><td>5000</td><td></td></tr><tr><td>Cryomold</td><td>VWR</td><td>18000-128</td><td></td></tr><tr><td>Curved forceps</td><td>Fine Science Tools</td><td>11003-14</td><td></td></tr><tr><td>Disposable polystyrene tube, 14 mL</td><td>Fisher Scientific</td><td>14-959-1B</td><td></td></tr><tr><td>Disposable Scalpel</td><td>Fisher Scientific</td><td>NC0595256</td><td></td></tr><tr><td>DNAse I</td><td>Worthington</td><td>LS002139</td><td></td></tr><tr><td>Dry ice</td><td>Airgas</td><td>N/A</td><td></td></tr><tr><td>Durmont #7Forceps</td><td>Fine Science Tools</td><td>11271-30</td><td></td></tr><tr><td>EDTA disodium salt dihydrate</td><td>Amresco</td><td>0105-500g</td><td></td></tr><tr><td>Ethanol, 100%</td><td>any</td><td>N/A</td><td></td></tr><tr><td>Fetal Bovine Serum (FBS)</td><td>Hyclone</td><td>SH30910.03</td><td></td></tr><tr><td>Filter top tube, 5 mL</td><td>VWR</td><td>352235</td><td></td></tr><tr><td>Fixable viability stain 780</td><td>Becton Dickinson</td><td>565388</td><td></td></tr><tr><td>Flow cytometer</td><td>Beckman Coulter</td><td>Gallios</td><td></td></tr><tr><td>Glucose</td><td>Fisher Chemical</td><td>D16-500</td><td></td></tr><tr><td>Goat anti-mouse IgG (488 conjugate)</td><td>Jackson immunoresearch</td><td>115-546-146</td><td></td></tr><tr><td>Goat anti-mouse IgG (594 conjugate)</td><td>Jackson immunoresearch</td><td>115-586-146</td><td></td></tr><tr><td>Goat anti-rabbit 488</td><td>Jackson immunoresearch</td><td>111-545-144</td><td></td></tr><tr><td>Goat anti-rat 594</td><td>Jackson immunoresearch</td><td>112-585-167</td><td></td></tr><tr><td>Goat anti-rat 650</td><td>Jackson immunoresearch</td><td>112-605-167</td><td></td></tr><tr><td>Hank&#039;s Balnced Salt Solution (HBSS)</td><td>Corning</td><td>21-020-CV</td><td></td></tr><tr><td>Hemacytometer</td><td>Andwin Scientific</td><td>02-671-51B</td><td></td></tr><tr><td>Hemostat</td><td>Fine Science Tools</td><td>13004-14</td><td></td></tr><tr><td>HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid)</td><td>ThermoFisher Scientific</td><td>15630080</td><td></td></tr><tr><td>KCl</td><td>Fisher chemical</td><td>BP366-500</td><td></td></tr><tr><td>KH&lt;sub&gt;2&lt;/sub&gt;PO&lt;sub&gt;4&lt;/sub&gt; (anhydrous)</td><td>Sigma Aldrich</td><td>P5655-100G</td><td></td></tr><tr><td>Liquid Nitrogen</td><td>Airgas</td><td>N/A</td><td></td></tr><tr><td>Mouse FC block (CD16/32)</td><td>Becton Dickinson</td><td>553141</td><td></td></tr><tr><td>Na&lt;sub&gt;2&lt;/sub&gt;HP0&lt;sub&gt;4&lt;/sub&gt; (anhydrous)</td><td>Fisher Chemical</td><td>S374-500</td><td></td></tr><tr><td>NaCl</td><td>Fisher chemical</td><td>S671-500</td><td></td></tr><tr><td>Needle, 25 gauge</td><td>Becton Dickinson</td><td>305122</td><td></td></tr><tr><td>Normal mouse serum</td><td>ThermoFisher Scientific</td><td>31881</td><td></td></tr><tr><td>Nylon mesh strainer</td><td>VWR</td><td>352350</td><td></td></tr><tr><td>OCT</td><td>Sakura</td><td>4583</td><td></td></tr><tr><td>Paraformaldehyde, 20%</td><td>Electron Microscopy Sciences</td><td>15713-S</td><td>Diluted to 4% using 1 x PBS</td></tr><tr><td>Pasteur pipette, 9 inch, unplugged</td><td>Fisher Scientific</td><td>13-678-20C</td><td></td></tr><tr><td>PBS (1x)</td><td>Corning</td><td>21-040-CV</td><td></td></tr><tr><td>PE Rat Anti-Mouse CD4</td><td>Becton Dickinson</td><td>553730</td><td></td></tr><tr><td>PE-CF594 Rat Anti-Mouse CD19</td><td>Becton Dickinson</td><td>562329</td><td></td></tr><tr><td>Percoll density gradient media</td><td>GE healthcare</td><td>17-0891-01</td><td></td></tr><tr><td>PerCP-Cy5.5 Rat Anti-Mouse CD45</td><td>Becton Dickinson</td><td>550994</td><td></td></tr><tr><td>Petri dish, 100 mm</td><td>VWR</td><td>353003</td><td></td></tr><tr><td>pH meter</td><td>Fisher Scientific</td><td>13-636-AB150</td><td></td></tr><tr><td>Pipet-Aid</td><td>Drummond Scientific Corporation</td><td>4-000-101</td><td></td></tr><tr><td>Pipette 200 &amp;micro;l</td><td>Gilson</td><td>FA10005M</td><td></td></tr><tr><td>Pipette tips, 1 mL</td><td>USA Scientific</td><td>1111-2831</td><td></td></tr><tr><td>Pipette tips, 200 &amp;micro;l</td><td>USA Scientific</td><td>1111-1816</td><td></td></tr><tr><td>Pipette, 1 mL</td><td>Gilson</td><td>FA10006M</td><td></td></tr><tr><td>Prolong Diamond mountant with DAPI</td><td>ThermoFisher Scientific</td><td>P36962</td><td></td></tr><tr><td>Purified Rat Anti-Mouse CD16/CD32</td><td>Becton Dickinson</td><td>553141</td><td></td></tr><tr><td>Rabbit anti-mouse CD3 (SP7 clone)</td><td>Abcam</td><td>ab16669</td><td></td></tr><tr><td>Rabbit anti-mouse laminin</td><td>Abcam</td><td>ab11575</td><td></td></tr><tr><td>Rat anti-mouse ERT-R7</td><td>Abcam</td><td>ab51824</td><td></td></tr><tr><td>RPMI 1640</td><td>Corning</td><td>10-040-CV</td><td></td></tr><tr><td>Serological pipet, 1 mL</td><td>VWR</td><td>357521</td><td></td></tr><tr><td>Serological pipet, 10 mL</td><td>VWR</td><td>357551</td><td></td></tr><tr><td>Serological pipet, 5 mL</td><td>VWR</td><td>357543</td><td></td></tr><tr><td>Sodium hydroxide</td><td>Fisher Scientific</td><td>S318-100</td><td></td></tr><tr><td>Sucrose</td><td>Fisher chemical</td><td>S5-500</td><td></td></tr><tr><td>Surgical scissors</td><td>Fine Science Tools</td><td>14001-16</td><td></td></tr><tr><td>Surgical scissors, extra fine</td><td>Roboz</td><td>RS-5882</td><td></td></tr><tr><td>Syringe, 10 mL</td><td>Becton Dickinson</td><td>302995</td><td></td></tr><tr><td>Syringe, 5 mL</td><td>Becton Dickinson</td><td>309646</td><td></td></tr><tr><td>Trypan blue</td><td>Gibco</td><td>15250-061</td><td></td></tr><tr><td>Vacuum filter system</td><td>Millipore</td><td>20207749</td><td></td></tr><tr><td>Vacuum flask</td><td>Thomas Scientific</td><td>5340-2L</td><td></td></tr><tr><td>Vacuum in-line filter</td><td>Pall Corporation</td><td>4402</td><td></td></tr><tr><td>Vacuum line</td><td>Cole Palmer</td><td>EW-06414-20</td><td></td></tr><tr><td>Water bath</td><td>ThermoFisher Scientific</td><td>Versa bath</td><td></td></tr></tbody></table>

isolating central nervous system tissues and associated meninges for the downstream analysis of immune cells

The central nervous system (CNS) is comprised of the brain and spinal cord and is enveloped by the meninges, membranous layers serving as a barrier between the periphery and the CNS. The CNS is an immunologically specialized site, and in steady state conditions, immune privilege is most evident in the CNS parenchyma. In contrast, the meninges harbor a diverse array of resident cells, including innate and adaptive immune cells. During inflammatory conditions triggered by CNS injury, autoimmunity, infection, or even neurodegeneration, peripherally derived immune cells may enter the parenchyma and take up residence within the meninges. These cells are thought to perform both beneficial and detrimental actions during CNS disease pathogenesis. Despite this knowledge, the meninges are often overlooked when analyzing the CNS compartment, because conventional CNS tissue extraction methods omit the meningeal layers. This protocol presents two distinct methods for the rapid isolation of murine CNS tissues (i.e., brain, spinal cord, and meninges) that are suitable for downstream analysis via single-cell techniques, immunohistochemistry, and in situ hybridization methods. The described methods provide a comprehensive analysis of CNS tissues, ideal for assessing the phenotype, function, and localization of cells occupying the CNS compartment under homeostatic conditions and during disease pathogenesis.

This representative experiment was aimed at quantifying B and T cells and describing B and T cell localization in the meningeal and parenchymal CNS compartments in homeostatic conditions as well as in a murine progressive MS model (i.e., TMEV-IDD). TMEV-IDD was induced in 5-week-old female SJL mice by intracranial infection with 5 x 106 plaque forming units (PFU) of TMEV BeAn as previously described29.
The present study assessed B and T cells in the meninges,...

Watch this Scientific Journal Video about Isolating Central Nervous System Tissues and Associated Meninges for the Downstream Analysis of Immune cells at JoVE.com

Isolating Central Nervous System Tissues and Associated Meninges for the Downstream Analysis of Immune cells

This paper presents two optimized protocols for examining resident and peripherally derived immune cells within the central nervous system, including the brain, spinal cord, and meninges. Each of these protocols helps to ascertain the function and composition of the cells occupying these compartments under steady state and inflammatory conditions.

The central nervous system (CNS) is comprised of the brain and spinal cord and is enveloped by the meninges, membranous layers serving as a barrier ...

isolating-central-nervous-system-tissues-associated-meninges-for

Research

JoVE Journal

Immunology and Infection

9.0K Views.  Dartmouth-Hitchcock Medical Center. This paper presents two optimized protocols for examining resident and peripherally derived immune cells within the central nervous system, including the brain, spinal cord, and meninges. Each of these protocols helps to ascertain the function and composition of the cells occupying these compartments under steady state and inflammatory conditions.

Video: Isolating Central Nervous System Tissues and Associated Meninges for the Downstream Analysis of Immune cells

Isolation of Brain and Spinal Cord Mononuclear Cells Using Percoll Gradients

Isolation of immune cells that infiltrate the central nervous system (CNS) during infection, trauma, autoimmunity or neurodegeneration, is often required to define their phenotype and effector functions. Histochemical approaches are instrumental to determine the location of the infiltrating cells and to analyze the associated CNS pathology. However, in-situ histochemistry and immunofluorescent staining techniques are limited by the number of antibodies that can be used at a single time to characterize immune cell subtypes in a particular tissue. Therefore, histological approaches in conjunction with immune-phenotyping by flow cytometry are critical to fully characterize the composition of local CNS infiltration. This protocol is based on the separation of CNS cellular suspensions over discontinous percoll gradients. The current article describes a rapid protocol to efficiently isolate mononuclear cells from brain and spinal cord tissues that can be effectively utilized for identification of various immune cell populations in a single sample by flow cytometry.

The current article describes a rapid protocol to efficiently isolate mononuclear cells from brain and spinal cord tissues that can be effectively utilized for flow cytometric analyses.

Isolation of immune cells that infiltrate the central nervous system (CNS) during infection, trauma, autoimmunity or neurodegeneration, is often required ...

Isolation of Mononuclear Cells from the Central Nervous System of Rats with EAE

Whether studying an autoimmune disease directed to the central nervous system (CNS), such as experimental autoimmune encephalomyelitis (EAE, 1), or the immune response to an infection of the CNS, such as poliomyelitis, Lyme neuroborreliosis, or neurosyphilis, it is often necessary to isolate the CNS-infiltrating immune cells.
In this video-protocol we demonstrate how to isolate mononuclear cells (MNCs) from the CNS of a rat with EAE. The first step of this procedure requires a cardiac perfusion of the rodent with a saline solution to ensure that no blood remains in the blood vessels irrigating the CNS. Any blood contamination will artificially increase the number of apparent CNS-infiltrating MNCs and may alter the apparent composition of the immune infiltrate. We then demonstrate how to remove the brain and spinal cord of the rat for subsequent dilaceration to prepare a single-cell suspension. This suspension is separated on a two-layer Percoll gradient to isolate the MNCs. After washing, these cells are then ready to undergo any required procedure. 
Mononuclear cells isolated using this procedure are viable and can be used for electrophysiology, flow cytometry (FACS), or biochemistry. If the technique is performed under sterile conditions (using sterile instruments in a tissue culture hood) the cells can also be grown in tissue culture medium. A given cell population can be further purified using either magnetic separation procedures or a FACS.

In this video we demonstrate how to isolate mononuclear cells from the central nervous system of rats with experimental autoimmune encephalomyelitis.

Whether studying an autoimmune disease directed to the central nervous system (CNS), such as experimental autoimmune encephalomyelitis (EAE, 1), or the ...

Biology

Reliable Isolation of Central Nervous System Microvessels Across Five Vertebrate Groups

Isolation of microvessels from the central nervous system (CNS) is commonly performed by combining cortical tissue from multiple animals, most often rodents. This approach limits the interrogation of blood-brain barrier (BBB) properties to the cortex and does not allow for individual comparison. This project focuses on the development of an isolation method that allows for the comparison of the neurovascular unit (NVU) from multiple CNS regions: cortex, cerebellum, optic lobe, hypothalamus, pituitary, brainstem, and spinal cord. Moreover, this protocol, originally developed for murine samples, was successfully adapted for use on CNS tissues from small and large vertebrate species from which we are also able to isolate microvessels from brain hemisphere white matter. This method, when paired with immunolabeling, allows for quantitation of protein expression and statistical comparison between individuals, tissue type, or treatment. We proved this applicability by evaluating changes in protein expression during experimental autoimmune encephalomyelitis (EAE), a murine model of a neuroinflammatory disease, multiple sclerosis. Additionally, microvessels isolated by this method could be used for downstream applications like qPCR, RNA-seq, and Western blot, among others. Even though this is not the first attempt to isolate CNS microvessels without the use of ultracentrifugation or enzymatic dissociation, it is unique in its adeptness for the comparison of single individuals and multiple CNS regions. Therefore, it allows for investigation of a range of differences that may otherwise remain obscure: CNS portions (cortex, cerebellum, optic lobe, brainstem, hypothalamus, pituitary, and spinal cord), CNS tissue type (gray or white matter), individuals, experimental treatment groups, and species.

The goal of this protocol is to isolate microvessels from multiple regions of the central nervous system of lissencephalic and gyrencephalic vertebrates.

Isolation of microvessels from the central nervous system (CNS) is commonly performed by combining cortical tissue from multiple animals, most often ...

Neuroscience

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing

As resident macrophages in the central nervous system, microglia actively control brain development and homeostasis, and their dysfunctions may drive human diseases. Considerable advances have been made to uncover the molecular signatures of homeostatic microglia as well as alterations of their gene expression in response to environmental stimuli. With the advent and maturation of single-cell genomic methodologies, it is increasingly recognized that heterogenous microglia may underlie the diverse roles they play in different developmental and pathological conditions. Further dissection of such heterogeneity can be achieved through efficient isolation of microglia from a given region of interest, followed by sensitive profiling of individual cells. Here, we provide a detailed protocol for the rapid isolation of microglia from different brain regions in a single adult mouse brain hemisphere. We also demonstrate how to use these sorted microglia for plate-based deep single-cell RNA sequencing. We discuss the adaptability of this method to other scenarios and provide guidelines for improving the system to accommodate large-scale studies.

We provide a protocol for isolation of microglia from different dissected regions of an adult mouse brain hemisphere, followed by semi-automated library preparation for deep single-cell RNA sequencing of full-length transcriptomes. This method will help to elucidate functional heterogeneity of microglia in health and disease.

As resident macrophages in the central nervous system, microglia actively control brain development and homeostasis, and their dysfunctions may drive ...

Dissection and Isolation of Murine Glia from Multiple Central Nervous System Regions

The methods presented here demonstrate laboratory procedures for the dissection of four different regions of the central nervous system (CNS) from murine neonates for the isolation of glial subpopulations. The purpose of the procedure is to dissociate microglia, oligodendrocyte progenitor cells (OPCs), and astrocytes from cortical, cerebellar, brainstem, and spinal cord tissue to facilitate further in vitro analysis. The CNS region isolation procedures allow for the determination of regional heterogeneity among glia in multiple cell culture systems. Rapid CNS region isolation is performed, followed by the mechanical removal of meninges to prevent meningeal cell contamination of glia. This protocol combines gentle tissue dissociation and plating on a specified matrix designed to preserve cell integrity and adherence. Isolating mixed glia from multiple CNS regions provides a comprehensive analysis of potentially heterogenous glia while maximizing the use of individual experimental animals. Additionally, following dissociation of regional tissue, mixed glia are further divided into multiple cell types including microglia, OPCs, and astrocytes for use in either single cell type, cell culture plate inserts, or co-culture systems. Overall, the demonstrated techniques provide a comprehensive protocol of broad applicability for careful dissection of four individual CNS regions from murine neonates and includes methods for the isolation of three individual glia cell types to examine regional heterogeneity in any number of in vitro cell culture systems or assays.

Here we present a protocol for in vitro isolation of multiple glial cell populations from a mouse CNS. This method allows for the segregation of regional microglia, oligodendrocyte precursor cells, and astrocytes to study the phenotypes of each in a variety of culture systems.

The methods presented here demonstrate laboratory procedures for the dissection of four different regions of the central nervous system (CNS) from murine ...

Isolating All CNS Resident Cell Types Simultaneously from Autoimmune Mice

Simultaneous Isolation of Principal Central Nervous System-Resident Cell Types from Adult Autoimmune Encephalomyelitis Mice

Experimental autoimmune encephalomyelitis (EAE) is the most common murine model for multiple sclerosis (MS) and is frequently used to further elucidate the still unknown etiology of MS in order to develop new treatment strategies. The myelin oligodendrocyte glycoprotein peptide 35-55 (MOG35-55) EAE model reproduces a self-limiting monophasic disease course with ascending paralysis within 10 days after immunization. The mice are examined daily using a clinical scoring system. MS is driven by different pathomechanisms with a specific temporal pattern, thus the investigation of the role of central nervous system (CNS)-resident cell types during disease progression is of great interest. The unique feature of this protocol is the simultaneous isolation of all principal CNS-resident cell types (microglia, oligodendrocytes, astrocytes, and neurons) applicable in adult EAE and healthy mice. The dissociation of the brain and the spinal cord from adult mice is followed by magnetic-activated cell sorting (MACS) to isolate microglia, oligodendrocytes, astrocytes, and neurons. Flow cytometry was used to perform quality analyses of the purified single-cell suspensions confirming viability after cell isolation and indicating the purity of each cell type of approximately 90%. In conclusion, this protocol offers a precise and comprehensive way to analyze complex cellular networks in healthy and EAE mice. Moreover, required mice numbers can be substantially reduced as all four cell types are isolated from the same mice.

Author Spotlight: Studying Neuroinflammatory Pathways Through Simultaneous Isolation of All Main CNS-Resident Cell Types 

To date, protocols for the simultaneous isolation of all principal central nervous system-resident cell types from the same mouse are an unmet demand. The protocol shows a procedure applicable in na&#239;ve and experimental autoimmune encephalomyelitis mice to investigate complex cellular networks during neuroinflammation and simultaneously reduce the required mice numbers.

Experimental autoimmune encephalomyelitis (EAE) is the most common murine model for multiple sclerosis (MS) and is frequently used to further elucidate ...

Obtaining Single-Cell Suspensions from Murine Brain and Skull Bone Marrow

Isolating Immune Cells from Mouse Brain and Skull

Mounting evidence indicates that the immune response triggered by brain disorders (e.g., brain ischemia and autoimmune encephalomyelitis) occurs not only in the brain, but also in the skull. A key step toward analyzing changes in immune cell populations in both the brain and skull bone marrow after brain damage (e.g., stroke) is to obtain sufficient numbers of high-quality immune cells for downstream analyses. Here, two optimized protocols are provided for isolating immune cells from the brain and skull bone marrow. The advantages of both protocols are reflected in their simplicity, speed, and efficacy in yielding a large quantity of viable immune cells. These cells may be suitable for a range of downstream applications, such as cell sorting, flow cytometry, and transcriptomic analysis. To demonstrate the effectiveness of the protocols, immunophenotyping experiments were performed on stroke brains and normal brain skull bone marrow using flow cytometry analysis, and the results aligned with findings from published studies.

Author Spotlight: Investigating the Complex Immune Response Following Brain Ischemia

To investigate the immune response to brain disorders, one common approach is to analyze changes in immune cells. Here, two simple and effective protocols are provided for isolating immune cells from murine brain tissue and skull bone marrow.

Mounting evidence indicates that the immune response triggered by brain disorders (e.g., brain ischemia and autoimmune encephalomyelitis) occurs not only ...

Isolation and Characterization of the Immune Cells from Micro-dissected Mouse Choroid Plexuses

The brain is no longer considered as an organ functioning in isolation; accumulating evidence suggests that changes in the peripheral immune system can indirectly shape brain function. At the interface between the brain and the systemic circulation, the choroid plexuses (CP), which constitute the blood-cerebrospinal fluid barrier, have been highlighted as a key site of periphery-to-brain communication. CP produce the cerebrospinal fluid, neurotrophic factors, and signaling molecules that can shape brain homeostasis. CP are also an active immunological niche. In contrast to the brain parenchyma, which is populated mainly by microglia under physiological conditions, the heterogeneity of CP immune cells recapitulates the diversity found in other peripheral organs. The CP immune cell diversity and activity change with aging, stress, and disease and modulate the activity of the CP epithelium, thereby indirectly shaping brain function. The goal of this protocol is to isolate murine CP and identify about 90% of the main immune subsets that populate them. This method is a tool to characterize CP immune cells and understand their function in orchestrating periphery-to-brain communication. The proposed protocol may help decipher how CP immune cells indirectly modulate brain function in health and across various disease conditions.

This study uses flow cytometry and two different gating strategies on isolated perfused mice brain choroid plexuses; this protocol identifies the main immune cell subsets that populate this brain structure.

The brain is no longer considered as an organ functioning in isolation; accumulating evidence suggests that changes in the peripheral immune system can ...

Isolating Tumor-Infiltrating Immune Cells from a Murine Brain

Source: Baker, G. J., et al., Lowenstein, P. R. <a href="https://app.jove.com/v/53676">Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells.</a> J. Vis. Exp. (2015)
The video demonstrates the isolation of tumor-infiltrating peripheral blood mononuclear cells from a mouse brain using mechanical dissociation and enzymatic digestion. This is followed by density gradient centrifugation to collect the immune cells for further analysis.

All procedures involving sample collection have been performed in accordance with the institute&#39;s IRB guidelines.
Prepare density centrifugation media ...

JoVE Encyclopedia of Experiments

Neuronal Culture Techniques

Co-culture and Interaction Studies

Preparing a Single-Cell Suspension of Immune Cells from Murine Brain Tissue

Source: DiSano, K. D., et al. <a href="https://app.jove.com/v/61166">Isolating Central Nervous System Tissues and Associated Meninges for the Downstream Analysis of Immune Cells.</a> J. Vis. Exp. (2020).
The video demonstrates the preparation of a single-cell suspension of immune cells from a murine brain tissue. It involves enzymatic digestion to release cells from the brain tissue, followed by density gradient centrifugation to isolate the immune cells based on their densities to obtain the single-cell suspension.

All procedures involving animal samples have been reviewed and approved by the appropriate animal ethical review committee. 
1. Preparing single-cell ...