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Dissection and Isolation of Murine Glia from Multiple Central Nervous System Regions

DOI :

10.3791/61345-v

8:00 min

June 4th, 2020

June 4th, 2020

2,666 Views

1Department of Neurosciences, Lerner Research Institute, Cleveland Clinic Foundation, 2Brain Health Research Institute, Kent State University

Here we present a protocol for in vitro isolation of multiple glial cell populations from a mouse CNS. This method allows for the segregation of regional microglia, oligodendrocyte precursor cells, and astrocytes to study the phenotypes of each in a variety of culture systems.

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Dissection

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