Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing

10.7K Views

09:49 min

December 3rd, 2019

DOI :

10.3791/60347-v

December 3rd, 2019


Explore More Videos

Microglia

Chapters in this video

0:05

Title

0:43

Brain Region Dissection

1:43

Mechanical Tissue Dissociation

2:40

Myelin Removal

4:25

Staining for Fluorescence-activated Cell Sorting

5:18

Index FACS Sorting

5:46

Single-cell RNA-sequencing Library Preparation

7:17

Results: Isolation of Region-Specific Microglia for Deep Single-Cell RNA Sequencing

9:16

Conclusion

Related Videos

article

10:06

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling

14.7K Views

article

09:52

One Mouse, Two Cultures: Isolation and Culture of Adult Neural Stem Cells from the Two Neurogenic Zones of Individual Mice

37.4K Views

article

06:18

Brain Slice Biotinylation: An Ex Vivo Approach to Measure Region-specific Plasma Membrane Protein Trafficking in Adult Neurons

12.8K Views

article

09:20

Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains

13.5K Views

article

06:38

Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing

18.5K Views

article

07:49

Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing (DIVA-Seq)

9.4K Views

article

09:41

Obtaining Human Microglia from Adult Human Brain Tissue

7.0K Views

article

07:10

Quantification of Global Histone Post Translational Modifications Using Intranuclear Flow Cytometry in Isolated Mouse Brain Microglia

1.5K Views

article

06:45

Isolation of Pure Astrocytes and Microglia from the Adult Mouse Spinal Cord For In Vitro Assays and Transcriptomic Studies

1.5K Views

article

02:30

Preparing Single-Cell Suspension from Mouse Brain to Study Immune Response to Brain Disorders

248 Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved