JoVE Logo
Faculty Resource Center

Sign In

MRM Microcoil Performance Calibration and Usage Demonstrated on Medicago truncatula Roots at 22 T

DOI :

10.3791/61266-v

10:22 min

January 16th, 2021

January 16th, 2021

5,065 Views

1Solid-state NMR, Leiden Institute of Chemistry, Faculty of Science, Leiden University, 2Laboratory of Biophysics, Wageningen University & Research, 3Laboratory of BioNanoTechnology, Wageningen University & Research, 4MAGNEtic resonance Facility, Wageningen University & Research, 5C.J. Gorter Center for High Field MRI, Radiology department, Leiden University Medical Centre, Leiden University, 6Institute for Medical Physics and Biophysics, Leipzig University

A protocol to study biological tissue at high spatial resolution using ultra-high field magnetic resonance microscopy (MRM) using microcoils is presented. Step-by-step instructions are provided for characterizing the microcoils. Finally, optimization of imaging is demonstrated on plant roots.

Tags

MRM Microcoil

-- Views

Related Videos

article

Loading Drosophila Nerve Terminals with Calcium Indicators

article

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

article

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

article

Swimming Performance Assessment in Fishes

article

Generation of Composite Plants in Medicago truncatula used for Nodulation Assays

article

Quantifying Agonist Activity at G Protein-coupled Receptors

article

An Improved Method for Accurate and Rapid Measurement of Flight Performance in Drosophila

article

MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules

article

High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications

article

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved