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CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

DOI :

10.3791/61367-v

7:25 min

June 9th, 2020

June 9th, 2020

8,750 Views

1Department of Biochemistry, Genetics & Microbiology, University of Pretoria, 2Forestry & Agricultural Biotechnology Institute (FABI), University of Pretoria

The CRISPR-Cas9 genome editing system is an easy-to-use genome editor that has been used in model and non-model species. Here we present a protein-based version of this system that was used to introduce a premature stop codon into a mating gene of a non-model filamentous ascomycete fungus.

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CRISPR Cas9 mediated Genome Editing

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