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CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans

DOI :

10.3791/58764-v

9:56 min

November 14th, 2018

November 14th, 2018

11,084 Views

1Department of Biology, Ball State University, 2Whitehead Institute for Biomedical Research

Efficient genome engineering of Candida albicans is critical to understanding the pathogenesis and development of therapeutics. Here, we described a protocol to quickly and accurately edit the C. albicans genome using CRISPR. The protocol allows investigators to introduce a wide variety of genetic modifications including point mutations, insertions, and deletions.

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CRISPR mediated Genome Editing

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