JoVE Logo
Faculty Resource Center

Sign In

Quantitative Approaches for Scoring in vivo Neuronal Aggregate and Organelle Extrusion in Large Exopher Vesicles in C. elegans

DOI :

10.3791/61368-v

9:06 min

September 18th, 2020

September 18th, 2020

6,965 Views

1Department of Molecular Biology and Biochemistry, Rutgers University

This protocol describes approaches for detection and quantitation of large aggregate and/or organelle extrusions (~4 µm) produced by C. elegans cells in the form of membrane-bound exophers. We describe strains, growth conditions, scoring criteria, timing, and microscopy considerations needed to facilitate dissection of this debris expulsion mechanism.

Tags

Quantitative Approaches

-- Views

Related Videos

article

Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation

article

In vivo Laser Axotomy in C. elegans

article

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI

article

Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices

article

In vivo Neuronal Calcium Imaging in C. elegans

article

Fast Micro-iontophoresis of Glutamate and GABA: A Useful Tool to Investigate Synaptic Integration

article

Super-resolution Imaging of Neuronal Dense-core Vesicles

article

Inducing Plasticity of Astrocytic Receptors by Manipulation of Neuronal Firing Rates

article

In Vivo Imaging of Dauer-specific Neuronal Remodeling in C. elegans

article

Simultaneous ex vivo Functional Testing of Two Retinas by in vivo Electroretinogram System

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved