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Quantitative Approaches for Scoring in vivo Neuronal Aggregate and Organelle Extrusion in Large Exopher Vesicles in C. elegans

DOI :

10.3791/61368-v

9:06 min

September 18th, 2020

September 18th, 2020

6,801 Views

1Department of Molecular Biology and Biochemistry, Rutgers University

This protocol describes approaches for detection and quantitation of large aggregate and/or organelle extrusions (~4 µm) produced by C. elegans cells in the form of membrane-bound exophers. We describe strains, growth conditions, scoring criteria, timing, and microscopy considerations needed to facilitate dissection of this debris expulsion mechanism.

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Quantitative Approaches

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