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In vivo Neuronal Calcium Imaging in C. elegans

DOI :

10.3791/50357-v

April 10th, 2013

April 10th, 2013

24,138 Views

1Department of Physiology and Biophysics, Boston University School of Medicine, 2Boston University Photonics Center

With its small transparent body, well-documented neuroanatomy and a host of amenable genetic techniques and reagents, C. elegans makes an ideal model organism for in vivo neuronal imaging using relatively simple, low-cost techniques. Here we describe single neuron imaging within intact adult animals using genetically encoded fluorescent calcium indicators.

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