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Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation

DOI :

10.3791/2678-v

April 17th, 2011

April 17th, 2011

15,168 Views

1Department of Neurobiology, Yale University, 2Program in Developmental Biology, Baylor College of Medicine

We demonstrate an in vivo electroporation protocol for transfecting single or small clusters of retinal ganglion cells (RGCs) and other retinal cell types in postnatal mice over a wide range of ages. The ability to label and genetically manipulate postnatal RGCs in vivo is a powerful tool for developmental studies.

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Transfection

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