JoVE Logo
Faculty Resource Center

Sign In

In vivo Laser Axotomy in C. elegans

DOI :

10.3791/2707-v

9:18 min

May 19th, 2011

May 19th, 2011

17,758 Views

1Department of Genetics, Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine

A protocol to cut neurons in C. elegans with a MicroPoint pulsed laser is presented. We describe setting up the system, immobilizing worms, and severing labeled neurons. Advantages include a relatively low-cost system and the ability to sever neuronal processes or ablate cells in vivo.

Tags

In Vivo Laser Axotomy

-- Views

Related Videos

article

Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation

article

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo

article

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI

article

Automated Quantification of Synaptic Fluorescence in C. elegans

article

C. elegans Tracking and Behavioral Measurement

article

A Molecular Readout of Long-term Olfactory Adaptation in C. elegans

article

In vivo Neuronal Calcium Imaging in C. elegans

article

Visualizing Neuroblast Cytokinesis During C. elegans Embryogenesis

article

Laser Nanosurgery of Cerebellar Axons In Vivo

article

In Vivo Imaging of Dauer-specific Neuronal Remodeling in C. elegans

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved