Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (Lactuca sativa L.) and Crop Wild Relatives (Lactuca spp.)
13.3K Views
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10:22 min
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June 30th, 2020
DOI :
June 30th, 2020
•1Unidad de Hortofruticultura, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), 2Unidad de Producción y Sanidad Animal, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), 3Dpto Química Analítica, Escuela Politécnica Superior (Universidad de Zaragoza), 4Instituto Agroalimentario de Aragón - IA2 (CITA-Universidad de Zaragoza)
Chapters in this video
0:04
Introduction
1:03
Plant Material Preparation
1:57
Ascorbic Acid (AA) Standard Preparation
2:34
AA and Dehydroascorbic Acid (DHAA) Extraction
3:40
DHAA Reduction for Total AA (TAA) Extraction
4:31
Ultra-High-Performance Liquid Chromatography-Ultraviolet Preparation
6:46
AA Determination
7:21
AA and DHAA Quantification
8:18
Results: Representative Vitamin C Quantification in Lactuca Species
9:44
Conclusion
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