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A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC)

DOI :

10.3791/55425-v

10:09 min

March 15th, 2017

March 15th, 2017

23,930 Views

1German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, 2Institute of Ecology, Friedrich Schiller University Jena

Here, we describe in great detail an established and robust protocol for the extraction of glucosinolates from ground plant materials. After an on-column sulfatase treatment of the extracts, the desulfoglucosinolates are eluted and analyzed by high-pressure liquid chromatography.

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Keywords Glucosinolate

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