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Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics

DOI :

10.3791/64792-v

9:09 min

December 9th, 2022

December 9th, 2022

3,864 Views

1Helmholtz Pioneer Campus (HPC), Helmholtz Munich, 2Institute of Computational Biology, Computational Health Department, Helmholtz Munich, 3Division of Infectious Diseases and Tropical Medicine, Ludwig-Maximillian-Universität Klinikum, 4TUM School of Medicine, Technical University of Munich

Here, we present a protocol for isolating nuclei from flash-frozen, archived liver tissues for single-nucleus RNA-seq, ATAC-seq, and joint multiomics (RNA-seq and ATAC-seq).

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Isolation Of Nuclei

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