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JoVE Journal

Cancer Research

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In Vitro Evaluation of Oncogenic Transformation in Human Mammary Epithelial Cells

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This methodology uses the integration of different indicators to assess the degree of transformation of any cell model. This technique provides researchers with a set of time-consuming but simple-to-use tools for assessing cell transformation in v

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This protocol provides experimental in vitro tools to evaluate the transformation of human mammary cells. Detailed steps to follow-up cell proliferation rate, anchorage-independent growth capacity, and distribution of cell lineages in 3D cultures with basement membrane matrix are described.

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