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Isolation, Proliferation and Differentiation of Rhesus Macaque Adipose-Derived Stem Cells

DOI :

10.3791/61732-v

May 26th, 2021

May 26th, 2021

1,851 Views

1Department of Physiology, Louisiana State University Health Sciences Center - New Orleans, 2Comprehensive Alcohol-HIV/AIDS Research Center, Louisiana State University Health Sciences Center - New Orleans

In this article, we describe isolation of rhesus macaque derived adipose-derived stem cells (ADSCs) using an enzymatic tissue digestion protocol. Next, we describe ADSC proliferation which includes cell detachment, counting and plating. Lastly, ADSC differentiation is described using specific adipogenic inducing agents. Additionally, we describe staining techniques to confirm differentiation.

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Isolation

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