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Isolation, Characterization, and Differentiation of Cardiac Stem Cells from the Adult Mouse Heart

DOI :

10.3791/58448-v

January 7th, 2019

January 7th, 2019

6,360 Views

1Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, 2Department of Anesthesiology, University of Nebraska Medical Center

The overall goal of this article is to standardize the protocol for the isolation, characterization, and differentiation of cardiac stem cells (CSCs) from the adult mouse heart. Here, we describe a density gradient centrifugation method to isolate murine CSCs and elaborated methods for CSC culture, proliferation, and differentiation into cardiomyocytes.

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