JoVE Logo
Faculty Resource Center

Sign In

Isolation, Characterization, and Differentiation of Cardiac Stem Cells from the Adult Mouse Heart

DOI :

10.3791/58448-v

January 7th, 2019

January 7th, 2019

6,388 Views

1Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, 2Department of Anesthesiology, University of Nebraska Medical Center

The overall goal of this article is to standardize the protocol for the isolation, characterization, and differentiation of cardiac stem cells (CSCs) from the adult mouse heart. Here, we describe a density gradient centrifugation method to isolate murine CSCs and elaborated methods for CSC culture, proliferation, and differentiation into cardiomyocytes.

Tags

Isolation

-- Views

Related Videos

article

Isolation of Retinal Stem Cells from the Mouse Eye

article

Isolation and Culture of Adult Epithelial Stem Cells from Human Skin

article

Isolation & Characterization of Hoechstlow CD45negative Mouse Lung Mesenchymal Stem Cells

article

Generation of Mice Derived from Induced Pluripotent Stem Cells

article

Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle

article

Naïve Adult Stem Cells Isolation from Primary Human Fibroblast Cultures

article

Isolation, Culture, and Functional Characterization of Adult Mouse Cardiomyoctyes

article

Manual Isolation of Adipose-derived Stem Cells from Human Lipoaspirates

article

Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor

article

Isolation of Enteric Glial Cells from the Submucosa and Lamina Propria of the Adult Mouse

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved