Results: Identification of FAPs and MPs via Flow Cytometry
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Conclusion
Transcript
As FAPs are mediators of muscle regeneration and pathological fibrosis, our protocol allows for characterization of FAP dynamics post muscle injury and isolation of FAPs for in vitro or ex vivo studies. To date, studies have been performed exclusi
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This protocol outlines a method to isolate Fibro-adipogenic progenitors (FAPs) and myogenic progenitors (MPs) from rat skeletal muscle. Utilization of the rat in muscle injury models provides increased tissue availability from atrophic muscle for the analysis and a larger repertoire of validated methods to assess muscle strength and gait in free-moving animals.