Purification of Exosomes Using Differential and Ultra-Centrifugation
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Results: Characterization of Purified Exosomes
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Conclusion
Transcript
The advantage of using differential centrifugation for this protocol is that it is easily scalable up to liters and therefore, ideal for isolating exosomes from primary fibroblasts. Purified exosomes can be obtained from different cell types and t
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This protocol illustrates the 1) the isolation and culture of primary fibroblasts from the adult mouse gastrocnemius muscle as well as 2) purification and characterization of exosomes using a differential ultracentrifugation method combined with sucrose density gradients followed by western blot analyses.