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Isolation and Characterization of Exosomes from Skeletal Muscle Fibroblasts

DOI :

10.3791/61127-v

May 16th, 2020

May 16th, 2020

2,921 Views

1Department of Genetics, St. Jude Children's Research Hospital

This protocol illustrates the 1) the isolation and culture of primary fibroblasts from the adult mouse gastrocnemius muscle as well as 2) purification and characterization of exosomes using a differential ultracentrifugation method combined with sucrose density gradients followed by western blot analyses.

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