The European Union&#39;s Horizon 2020 Research and Innovation Programme under the Marie Sk&#322;odowska-Curie Grant with the grant agreement no. 765602 funded this work. All authors recognize the support of the Adolphe Merkle Foundation and the University of Ferrara&#160;and gratefully acknowledge Dow Silicones Belgium. Dr. Miguel Spuch-Calvar is acknowledged for preparing the graphical images of the RhE cultivation process. The authors thank Dr. Barbara Drasler, Dr. Franco Cervellati, and Dr. Agn&#232;s Tessier for their technical support and discussions.

Review and adhere to national and international ethical considerations and conditions related to the use of human tissues or cells before planning and executing any research activity involving this protocol. 
NOTE: All steps of this protocol must be carried out in aseptic conditions. Biosafety Level 2 practices are the minimum requirement for the cultivation of RhEs. All necessary safety precautions must be taken when handling the chemicals/reagents described in this protocol.
1. Preparation of cell culture media
NOTE: There are three different types of serum-free culture media used for the cultivation of RhEs (Table 1): (i) the basal medium with low calcium level (60 &#181;M Ca2+) used for the 2D culture of NHEKs; (ii) the submerged medium with high calcium level (1.5 mM Ca2+) used for the seeding of NHEKs into the cell culture insert system; and (iii) the air-liquid interface (ALI) medium with high calcium level (1.5 mM Ca2+), ascorbic acid, and keratinocyte growth factor&#160;(KGF).
<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>Medium</td>
			<td>Medium information</td>
			<td>Required quantity</td>
		</tr>
		<tr>
			<td rowspan="3">Basal medium</td>
			<td>Keratinocyte growth medium</td>
			<td rowspan="3">36 mL/24 wells</td>
		</tr>
		<tr>
			<td>+ 1 % [v/v] HKGS</td>
		</tr>
		<tr>
			<td>+ 1 % [v/v] 100x antibiotic-antimycotic</td>
		</tr>
		<tr>
			<td rowspan="4">Submerged medium</td>
			<td>Keratinocyte growth medium</td>
			<td rowspan="4">36 mL/24 wells</td>
		</tr>
		<tr>
			<td>+ 1 % [v/v] HKGS</td>
		</tr>
		<tr>
			<td>+ 1 % [v/v] 100x antibiotic-antimycotic&#160;</td>
		</tr>
		<tr>
			<td>+ 1.5 mM Ca2+</td>
		</tr>
		<tr>
			<td rowspan="6">Air-liquid interface medium</td>
			<td>Keratinocyte growth medium</td>
			<td rowspan="6">216 mL/24 wells</td>
		</tr>
		<tr>
			<td>+ 1 % [v/v] HKGS</td>
		</tr>
		<tr>
			<td>+ 1 % [v/v] 100x antibiotic-antimycotic&#160;</td>
		</tr>
		<tr>
			<td>+ 1.5 mM Ca2+</td>
		</tr>
		<tr>
			<td>+ 50 &#181;g/mL ascorbic acid</td>
		</tr>
		<tr>
			<td>+ 10 ng/mL keratinocyte growth factor</td>
		</tr>
	</tbody>
</table>
Table 1.&#160;Summary table of the different culture media used to cultivate RhEs. List of different culture media with supplements.
<ol>
	<li>Prepare the basal medium.
	<ol>
		<li>Supplement the bottle of 500 mL of keratinocyte growth medium (Table of Materials) with 5 mL of human keratinocyte growth supplements (HKGS) in order to reach final concentrations of 0.2% [v/v] bovine pituitary extract (BPE), 0.2 ng/mL human recombinant epidermal growth factor (EGF), 0.18 &#181;g/mL hydrocortisone, 5 &#181;g/mL bovine transferrin, and 0.01 &#181;g/mL of recombinant human insulin-like growth factor-I (IGF-I).</li>
		<li>Add 5 mL of 100x antibiotic-antimycotic solution containing 10,000 units/mL of penicillin, 10,000 &#181;g/mL of streptomycin, and 25 &#181;g/mL of amphotericin B.</li>
	</ol>
	</li>
	<li>Prepare the submerged medium.
	<ol>
		<li>Supplement the bottle of 500 mL of keratinocyte growth medium (Table of Materials) with 5 mL of HKGS in order to reach final concentrations of 0.2% [v/v] BPE, 0.2 ng/mL human recombinant EGF, 0.18 &#181;g/mL hydrocortisone, 5 &#181;g/mL bovine transferrin, and 0.01 &#181;g/mL of human recombinant IGF-I.</li>
		<li>Add 5 mL of 100x antibiotic-antimycotic solution.</li>
		<li>Add 5 mL of a 0.144 M CaCl2 (calcium chloride)&#160;stock solution to reach a final concentration of 1.5 mM Ca2+. 
		NOTE:&#160;The calcium concentration is already increased during the submerged phase to stimulate the differentiation of the keratinocytes and initiate the stratification process49.</li>
	</ol>
	</li>
	<li>Prepare the air-liquid interface (ALI) medium.
	<ol>
		<li>Supplement one bottle of 500 mL of keratinocyte growth medium (Table of Materials) with 5 mL of HKGS in order to reach final concentrations of 0.2% [v/v] BPE, 0.2 ng/mL human recombinant EGF, 0.18 &#181;g/mL hydrocortisone, 5 &#181;g/mL bovine transferrin, and 0.01 &#181;g/mL of human recombinant IGF-I.</li>
		<li>Add 5 mL of 100x antibiotic-antimycotic solution.</li>
		<li>Add 5 mL of a 0.144 M CaCl2 stock solution to reach a final concentration of 1.5 mM Ca2+.</li>
		<li>Add 1 mL of a 25 mg/mL ascorbic acid stock solution to reach a final concentration of 50 &#181;g/mL ascorbic acid.</li>
		<li>Add 50 &#181;L of a 100 &#181;g/mL KGF in 1% [w/v] bovine serum albumin in phosphate-buffered saline (PBS) stock solution to reach a final concentration of 10 ng/mL KGF. 
		&#8203;NOTE: Since ascorbic acid is sensitive to oxidation, it is recommended to use a stable ascorbic acid-derivative, such as magnesium l-ascorbyl-2-phosphate59 or L-ascorbic acid 2-phosphate sesquimagnesium60. If ascorbic acid is used, it is recommended to freshly supplement the ALI medium with ascorbic acid before each refresh.</li>
	</ol>
	</li>
</ol>
2. Culture of NHEKs
NOTE: Since primary human keratinocytes remain proliferative upon their fourth or fifth passage61, NHEKs in their third passage are used for the cultivation of RhEs. Primary keratinocytes should be handled very carefully due to their high sensitivity. Careful and slow pipetting of cell suspensions at any time is very important, to not disturb the condition of the cells.
<ol>
	<li>Thaw a vial with 1 x 106 cryopreserved NHEKs in a water bath at 37 &#176;C, by submerging part of the vial in the water. Incubate the vial for 1-2 minutes in the water bath, until only a small sliver of ice is visible. 
	CAUTION: Do not submerge the whole vial in the water bath to avoid contaminations. Do not thaw the cells longer than 2 minutes; this can reduce the cell viability. Wipe the vial with a 70% ethanol solution before transferring the tube into the laminar hood.</li>
	<li>Resuspend the cells very carefully, by pipetting up and down 2-3 times. Transfer the cell suspension into two T75 flasks containing a total of 15 mL of pre-warmed thawing medium, resulting in a seeding density of 6.7 x 104 cells/cm2. 
	NOTE: For the first two passages and the thawing of cryopreserved NHEKs, cell culture medium is used according to the supplier&#39;s recommendations.</li>
	<li>Place the flasks into the cell culture incubator (37 &#176;C, 5% CO2, and 95% relative humidity (RH)).</li>
	<li>After approximately 24 hours, replace the thawing medium by the basal medium to remove dimethyl sulfoxide (DMSO) from the keratinocyte freezing solution.</li>
	<li>Refresh the basal medium every two days.</li>
	<li>After 4-6 days of cultivation, the cells should be around 80% confluent and ready for seeding in inserts for the cultivation of RhEs. 
	&#8203;NOTE: Keratinocytes should be grown to maximum 80% confluence to preserve their proliferative capacity62. The number of cells to be thawed must take into consideration several parameters, such as the cell passage number, the cell viability upon thawing, the seeding efficiency as well as the doubling time.</li>
</ol>
3. Seeding of NHEKs
NOTE: This protocol is designed for use within a 24-well carrier plate format. If other plate formats are required (e.g., 12-well or 6-well format), optimizations in the seeding density and medium volume should be considered. Figure 1 summarizes a proposed timeline for RhE cultivation and shows the cultivation conditions.
<img alt="Figure 1" class="xfigimg" src="/files/ftp_upload/61802/61802fig1v4.jpg" /> 
Figure 1: Schematic timeline of the reconstitution protocol.&#160;Presentation of the&#160;RhE model preparation, cultivation process, and application (exposure to chemical substance). The scheme includes the appropriate cell culture media types for each step.&#160;<a href="https://www.jove.com/files/ftp_upload/61802/61802fig1v4large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<ol>
	<li>Pre-fill 24-well plates with 1.5 mL of submerged medium, ideally using a dispenser pipette.</li>
	<li>Remove the basal medium from the T75 flasks used for the culture of the NHEKs.</li>
	<li>Rinse the cells by adding 5 mL of pre-warmed PBS&#160;to each T75 flask.</li>
	<li>Remove PBS from the flasks. 
	NOTE: This step is crucial, since the medium contains proteins and calcium that will inhibit the trypsin activity.</li>
	<li>Add 2-3 mL of pre-warmed 0.05% [v/v] trypsin/ethylene diamine tetra acetic acid (EDTA) to each T75 flask. Make sure that the trypsin solution is equally distributed on the cell culture area of the flask. 
	CAUTION: The 2 mL volume is based on the 80% confluence mentioned above. Use 3 mL for flasks with a higher confluency.</li>
	<li>Place the flasks for 4 minutes in the cell culture incubator (37 &#176;C, 5% CO2, and 95% RH). Check whether the cells detach using the microscope at a 10x magnification. Rap the flask against the palm of the hand to help the cells release from the surface of the flask. Detached cells can be observed as rounded cells floating in the trypsin solution. 
	CAUTION: Do not incubate the cells in trypsin for longer than 6 minutes. Over-trypsinization can damage the cells and decrease their adherence63.</li>
	<li>Once all the cells are detached, add an equal volume (i.e., 2-3 mL) of pre-warmed trypsin inhibitor to each T75 flask.</li>
	<li>Transfer the cell suspension from the flasks to a centrifuge tube.</li>
	<li>Rinse the flasks with 5 mL of pre-warmed PBS and transfer it to the centrifuge tube containing the cell suspension. 
	NOTE: Make sure that most of the cells are collected by checking the number of residual cells in the flasks under the microscope. The surface of the flask should be 95% empty. If this is not the case it is possible to repeat the trypsinization step (3.3-3.9). Note however that re-trypsinization should be avoided.</li>
	<li>Centrifuge the harvested cells at 400 x g for 5 min.</li>
	<li>Carefully discard most of the supernatant, leaving approximately 100-200 &#181;L in the tube. 
	CAUTION: Do not aspirate the pellet during this procedure.&#160;</li>
	<li>Gently resuspend the pellet of cells in a low volume of submerged medium, pipette up and down 5-10 times to ensure a uniform cell suspension. Start with a low volume (i.e., 500 &#181;L) to avoid the formation of cell aggregates and add up to 1 mL of submerged medium in total per initial T75 flask. 
	NOTE: Gently flick the tube with fingers to carefully dissolve a part of the cell pellet in the supernatant.</li>
	<li>Count the cells in the suspension using the trypan blue exclusion method.
	<ol>
		<li>Dilute 0.4% [v/v] trypan blue stain and the cell suspension in a 1:1 ratio, by adding 10 &#181;L of 0.4% [v/v] trypan blue stain to 10 &#181;L of cell suspension. Add 10 &#181;L of the solution to a counting slide. Measure the cell count immediately after mixing the cell suspension with trypan blue, since trypan blue starts to decrease cell viability after exposure longer than 1 min65. 
		&#8203;CAUTION: Trypan blue was shown to be a potential mutagen, carcinogen, and teratogen64. Handle the dye with care and dispose of the waste safely according to local laboratory regulations. 
		NOTE: An alternative approach to the use of trypan blue is the non-hazardous dye Erythrosin B66.</li>
	</ol>
	</li>
	<li>Dilute the cell suspension with additional submerged medium to reach a concentration of 3.525 x 105 cells/mL in submerged medium by adding the volume V2 as shown in equation 1: 
	<img alt="Equation 1" src="/files/ftp_upload/61802/61802eq01.jpg" /> 
	C1 = counted cell concentration in the cell suspension obtained in 3.12 (cells/mL) 
	V1 = volume used to resuspend the pellet of cells in 3.12 (mL) 
	C2 = targeted cell concentration in the suspension (i.e., 3.525&#160;x 105 cells/mL) 
	V2 = volume to be added to reach the targeted cell concentration (mL) 
	NOTE: The surface area of the recommended culture insert is 0.47 cm2; therefore, the corresponding seeding density is 3.75 x 105 cells/cm2.</li>
	<li>Perform a second cell count (C3) of the diluted solution obtained in step 3.14. Use equation 2 to calculate the cell suspension volume (V4) to be seeded into the culture insert: 
	<img alt="Equation 2" src="/files/ftp_upload/61802/61902eq02.jpg" /> 
	C3 = targeted cell concentration in the suspension (i.e., 3.525&#160;x 105 cells/mL) 
	V3 = targeted volume of the cell suspension to be seeded in the culture insert (i.e., 0.5 mL) 
	C4 = counted cell concentration in the diluted suspension obtained in 3.14 (cells/mL) 
	V4 = actual volume of the cell suspension to be seeded in the culture insert (mL)</li>
	<li>Hang the 24 cell culture inserts in the highest position of the recommended carrier plate and transfer the carrier plate to the 24-well plate pre-filled with submerged medium (cf. 3.1). 
	CAUTION: When transferring the carrier plate to the multi-well plate, ensure that no air bubbles are trapped between the insert membrane and the submerged medium from the basal compartment, as this will affect the feeding of the cells and ultimately compromise the RhE viability and morphology.</li>
	<li>Add the determined volume V4 (from equation 2) of the cell suspension to each insert. 
	NOTE: It is recommended&#160;to use the reverse pipetting technique to accurately dispense the cell suspension to the culture inserts. 
	CAUTION: Make sure not to damage the membrane when dispensing the cell suspension into the culture insert. A precaution is to dispense the cell suspension along the wall of the insert system without touching the surface of the membrane.</li>
	<li>After seeding, incubate the 24-well plates for 10-15 min at room temperature, to overcome an edge effect (i.e., non-uniform temperature distribution between all wells67). Do not move the plates during this time.</li>
	<li>Transfer the plates to the cell culture incubator (37 &#176;C, 5% CO2, and 95% RH). The cells are maintained&#160;in submerged conditions for three days. 
	&#8203;NOTE: To avoid tissue variability, do not stack the plates in the incubator after seeding to make sure that each insert receives the same amount of heat. After three days (i.e., during&#160;ALI cultivation), stacking of plates is possible.</li>
</ol>
4. Cultivation at Air-Liquid Interface
<ol>
	<li>After a three-day incubation in the cell culture incubator (37 &#176;C, 5% CO2, and 95% RH), expose the cells that have adhered to the membrane surface to the ALI by removing&#160;the submerged medium from the apical compartment preferably using an aspiration system and a glass Pasteur pipette. 
	NOTE: Alternatively, the submerged medium from the apical compartment can be removed with a manual micropipette.</li>
	<li>Fill new 24-well plates with 1.5 mL of fresh pre-warmed ALI medium and transfer the carrier plates with the culture inserts to the new multi-well plates.</li>
	<li>Transfer the multi-well plates back to the cell culture incubator (37 &#176;C, 5% CO2, and 95% RH).</li>
	<li>Refresh the ALI medium every 2-3 days for 14 days.</li>
	<li>Perform the refresh in two steps: 1) prepare a new plate containing 1.5 mL/well of fresh pre-warmed ALI medium and 2) transfer the carrier plate to the new plate. 
	CAUTION: During the entire reconstitution procedure it is best not to remove the lid covering the carrier plate to keep the RhEs protected from potential contamination. 
	NOTE: The ALI step is crucial for the development of a stratified epidermal model as it allows terminal differentiation of the keratinocytes68. After going to ALI, a visual control of the inserts is required, to check whether there are &#39;leaky tissues&#39;: medium droplets on the tissue surface coming from&#160;the basolateral compartment. If the leakage happens at ALI day 3, gently remove the medium from the culture insert without touching the surface of the tissue. If the leakage&#160;persists, it is recommended to discard leaking&#160;tissues as it is an indication that there is no correct barrier formation in the RhE model.</li>
	<li>At the end of the reconstitution process, the tissues can be exposed to various stressors to induce for example oxidative stress or inflammation. In parallel, they can be treated with chemical compounds or cosmetic ingredients. NOTE: During the exposure/treatment, tissues are usually maintained in submerged medium starting from ALI D14. When tissues are expected to be exposed/treated for a long period of time (i.e., 48-72 hours), it is recommended (i) to start the exposure/treatment earlier in the ALI cultivation process, such as D7-D9, to avoid the thinning of the viable layers and the thickening of the SC, and (ii) to incubate the tissues in ALI medium, to keep stimulating cell proliferation.&#160;</li>
	<li>For RhE harvesting, collect the tissues&#160;and cell culture medium&#160;at the timepoint of interest&#160;for histological analysis, viability assays, protein/RNA extraction, and enzyme-linked immunosorbent assays (ELISAs).</li>
</ol>

Marc Eeman and Benedetta Petracca are employees of Dow Silicones Belgium. All other authors have nothing to disclose.

RhEs are widely used as screening tools in the pharmaceutical and dermato-cosmetic fields36, 75,76,77,78. Although several companies have made such RhEs commercially available, they remain costly and limit the possibility to vary cultivation parameters as required to address&#160;new research questions. This paper describes the production procedure of in-house RhEs in a robust and reliable manner and provides a detailed characterization of the obtained tissues to confirm the relevance of the model as an alternative approach to animal testing.
Some of the steps in the protocol are crucial to assure proper&#160;keratinocyte&#160;differentiation and RhE reproducibility. This can be carried out by utilizing the optimal cells, medium type(s), and cultivation conditions. In the proposed RhE model, neonatal NHEKs were selected for their lack of antigenic exposure, compared to adult NHEKs. Furthermore, keratinocytes were limited to Caucasian ethnicity to avoid inter-species variability. Primary keratinocytes are typically used for their ability to differentiate and stratify79. They can be obtained commercially or by in-house isolation from adult skin80. The cultivation of a cell line (i.e., HaCaT) on a polycarbonate membrane, has shown to fail differentiation and demonstrated an impaired capacity to synthesize lipids that are necessary for barrier formation81. However, the inclusion of different culture matrices, such as hydrogels, collagen, fibrin, and spheroids cultures, resulted in the successful development of 3D skin models78,82,83,84,85,86. The immortalized cell lines, N/TERT, have been shown to be suitable for the development of RhEs35. Primary keratinocytes remain proliferative upon their fourth or fifth passage61, therefore the current protocol includes the use of&#160;keratinocytes in their third passage. De Vuyst et al. have demonstrated that the cell seeding density is of importance and needs to be sufficient (i.e., &#8805; 2.5x105 cells/cm2) to ensure that the medium from the basolateral compartment does not diffuse to the apical compartment. An insufficient seeding density (i.e., &#60; 2.5x105 cells/cm2) can result in an inability to form a proper barrier, which is indicated by the diffusion of medium from the basolateral to the apical compartment, resulting in submerged instead of ALI culture conditions62. Serum-free keratinocyte growth medium&#160;(see Table of Materials) was preferred for reproducibility purposes, since it offers the advantage of working with a chemically defined medium and reduces the risk of contamination. This medium has a lower calcium concentration (i.e., 60 &#181;M) and therefore stimulates the proliferation of keratinocytes87. Increasing the calcium concentration (i.e., 1.5 mM) from the first step of the RhE cultivation, favors&#160;keratinocyte differentiation and skin barrier formation and homeostasis49. In addition, the ALI medium is supplemented with ascorbic acid, which has shown to be crucial for the formation of SC lipids and to promote differentiation52,53,54. The ALI medium also contains KGF, which is a growth factor secreted by fibroblasts that can bind to keratinocyte transmembrane receptors and upon activation has a dual role in differentiation and wound repair55, 88. It is important to refresh the ALI medium at specific time intervals, to provide a constant supply of fresh nutrients to the RhEs. The use of a carrier plate system is crucial for RhE cultivation at a larger scale (i.e., 24 inserts/plate). It offers the advantage of saving time, reducing the risk of contamination, and leaves less room for the introduction of human errors. It also provides the possibility to culture RhEs in a high volume of media (i.e., 1.5 mL), which reduces the required number of ALI medium refreshes. Additionally, it offers the possibility to transfer a full plate of inserts to a plate with fresh medium, avoiding contact with the inserts individually or uncovering the plate lid.
There are several limitations of the RhE model that should be noted. In native human skin there is an equilibrium between the proliferation of keratinocytes in the basal layer and the detachment of corneocytes in the SC (i.e., desquamation)89. However, in vitro, desquamation does not take place. Therefore, the corneocytes remain attached to the RhE and form a thick SC that is less physiologically relevant. Hence, there is a limited cultivation timespan of RhEs. Moreover, this RhE model is simple and straightforward, since it consists of a singular cell type, i.e., the keratinocyte, which is the most abundant cell type of the epidermis. However, there are other cell types resident in the epidermis, such as melanocytes, dendritic cells (i.e., Langerhans cells), T cells (e.g., CD8+ cells), and Merkel cells13. To enhance the physiological relevance of the skin model, researchers have made skin models more complex by adding melanocytes38 , immune cells39, or patient-derived cells90. One should keep in mind that the barrier properties of human skin models are different compared to native human skin, due to notably a different SC lipid composition and a higher barrier permeability 91,92,93,94,95. However, several studies have reported&#160;changes in barrier properties of human skin models by the cultivation under hypoxia96 or decreased relative humidity97, the modulation of the dermal matrix with chitosan98, and alteration in the free fatty acids in the culture medium99. Moreover, in both simple and more complex RhEs, the culture conditions and medium composition can be modulated to mimic pathological features. By challenging the model with cytokines, an abnormal morphology100 and alterations in gene and protein expression levels can be established that are typically observed in common skin disorders, such as atopic dermatitis and psoriasis35, 101,102,103,104,105. Silencing specific genes in keratinocytes&#160;before initiating the reconstitution process of the 3D model is another approach used to mimic features of skin disorders and investigate new therapeutic solutions106, 107. Besides modeling an epidermal layer only, a dermal compartment can be included in the model (i.e., named human skin equivalents or full-thickness models) by embedding fibroblasts in a collagen matrix prior to RhE reconstitution, making it more physiologically relevant and suitable for aging and wound healing related studies108,109,110. Additionally, tumor spheroids have been added to human skin equivalents to study melanoma progression111, 112. The latest advances in the field of skin models are bio-printing and skin-on-a-chip. Multiple research groups have succeeded recently in the development of (perfusable) bio-printed skin equivalents45, 113, 114. The proposed protocol takes advantage of a 24-well format and a carrier plate, avoiding inserts to be handled individually. However, the study scale is still quite limited and lacks automation. By implementing the use of bio-printing or skin-on-a-chip, smaller skin models can be used with more automated processes and on a larger scale.
The RhE described in this protocol has multiple similarities to the already developed and well characterized commercial epidermal models. The morphological analysis has demonstrated that although the number of viable layers in the proposed RhE model is lower compared to that of native human skin (i.e., 6-7 compared to 7-14), it is comparable to that of the EpiDerm RhE model (i.e, 8-12)70. Similarly to the EpiDerm, EpiSkin, and SkinEthic models, the upper RhE layer shows a basket-weave pattern of densely packed corneocyte layers28. Moreover, TEM analysis revealed that the number of SC layers in the proposed RhE model (i.e., 15-25) is comparable to that of EpiDerm (i.e., 16-25)70. Overall, the proposed RhE model demonstrates a similar structure to that of other commercialized epidermal models, mimicking the native human epidermis. The tissue integrity as measured by TEER is in range with commercial RhE models (i.e., between 3000-5600 &#937;.cm2)71,72,73 and other in-house RhEs (i.e., approximately 5000 &#937;.cm2)74, 115. The proposed RhE model shows to be well differentiated, as indicated by the presence and correct localization of differentiation and tissue adhesion markers. Moreover, the proposed RhE model shows to be responsive to proinflammatory stimuli (i.e., LPS and TNF-&#945;).
To conclude, the current protocol shows how to produce RhEs in a reliable manner and at a relatively large scale to meet the needs of researchers in both academic and private institutions. The proposed RhE model shows to have similar morphology, epidermal differentiation, and biological responsiveness to other existing commercial models. It provides an alternative tool for both the pharmaceutical and dermato-cosmetic field when access to a relevant skin model is required.

The epidermis is the outermost layer of the skin, at the direct interface between the human body and the external environment. Its main functions are to provide protection and hydration1. The epidermis acts as an effective physical barrier against external agents and prevents excessive water loss from the body. These skin functions mainly depend on the cellular arrangement in the outermost layers of the skin, the composition, and organization of intercellular lipids2. The epidermis is primarily composed of keratinocytes that migrate upwards to the outer side of the tissue and undergo differentiation. There are 4-5 epidermal layers that are characterized by their stage of differentiation. From the inside to the outside, the epidermal layers start from the viable epidermis, i.e., the stratum basale (SB), the stratum spinosum (SS), and the stratum granulosum (SG), to the non-viable uppermost layer, i.e., the stratum corneum (SC)3. The basal layer is mainly composed of proliferating keratin-enriched keratinocytes, which migrate through the SS upon differentiation4. During keratinocyte maturation, various changes in protein expression and structure occur. Keratinocytes adhere through the formation of desmosomal junctions5. In the SG, the generation of lamellar bodies is initiated. They consist&#160;of lipid precursors and enzymes that are crucial for the formation of the skin barrier function6. The SG is also characterized by the presence of keratohyalin granules in the cytoplasm of the keratinocytes. At the interface with the SC, the content&#160;of the lamellar bodies is&#160;extruded into the intercellular spaces and the non-polar lipids such as ceramides, cholesterol, and free fatty acids organize into stacked lamellar lipid bilayers to form the extracellular lipid matrix7. In the SC, cells lose all cellular organelles including the nucleus, due to enzymatic degradation processes and adopt a flattened morphology. They are surrounded by a cornified envelope made of cross-linked protein layers, and are referred to as corneocytes8, 9. Desmosomal components are cross-linked to the cornified envelope to form corneodesmosomes and bind the corneocytes together. The resulting epithelium is continually renewed from stem cells, with a turnover time of approximately 5-6 weeks10. The differentiation process of the keratinocytes, which results in a fully stratified epidermis, is crucial for the formation of the barrier function of the skin11.
During wounding and inflammation, keratinocytes induce changes in adhesion molecules and surface receptors and trigger proinflammatory responses via secretion of cytokines, chemokines, and antimicrobial peptides12. The skin is not only a physical barrier against exogenous substances; it also acts as an immune sensor upon exposure to pathogens. In addition, it regulates the diffusion of several substances across its layers, such as water content to protect the human body from dehydration. The skin is also involved in the synthesis of vitamin D and has various other metabolic functions3, 13, 14.
To assess the adverse effects of exogenous substances, toxicologists have relied for decades on animal testing, but nowadays it is not the preferred approach. Besides having limited predictive capacity for human toxicity, animal models involve numerous ethical issues. The ban on animal testing in the cosmetic industry and the recommendation to follow the 3R principle&#160;(i.e., Replacement, Reduction, and Refinement) in research have led to the development of alternative test methods based on in vitro approaches15.The first in vitro skin cell models have already been described in the 90&#39;s, and an impressive development from simple human keratinocyte mono-cultures to fully differentiated epidermis and full-thickness models&#160;has been achieved16. Nowadays, skin tissue engineering has gained importance in both the pharmaceutical and dermato-cosmetic fields. In the last two decades, several companies have commercialized three-dimensional (3D) reconstructed human epidermis (RhE) that represent standardized and reproducible tools for skin-related studies. Several commercial RhE models are accepted for in vitro skin testing of chemicals according to OECD guidelines for the testing of skin irritation17, 18 (i.e., test guideline 43919) and skin corrosion20 (i.e., test guideline 43121). The in vitro test for skin sensitization22 (i.e., SENS-IS assay) is currently in the approval track and under peer-review23. There are also numerous other assays developed that utilize commercial RhE models, to evaluate phototoxicity24, to test drug formulations25, cosmetic formulations and active ingredients26, to study the skin barrier function27 and to test the biological response to environmental stressors28,29,30,31.
In addition to commercially available 3D skin models, multiple research groups have developed their own RhEs32,33,34,35,36,37. In-house RhEs offer the advantage for controlling the culture conditions according to the purpose of the study. Specifically, researchers can select the type and the source of the keratinocytes to be used for the reconstitution of their 3D epidermal model (i.e., primary vs. immortalized, neonatal vs. aged, single vs. pooled random donors, sex, ethnicity, individual living habits such as smoking, etc.). They have the possibility to vary the composition of the culture medium and incorporate growth factors, vitamins, or other compounds that can modulate the expression of target proteins or lipids. With in-house RhEs, researchers can also investigate biological responses and biomechanical properties as a function of the differentiation state of the 3D model. In addition to those intuitive parameters, there are continuous efforts to increase the complexity of 3D skin models and make them more physiologically relevant, for instance by adding other epidermal cell types (e.g. melanocytes and immune cells)38, 39, by culturing the keratinocytes on top of a fibroblast-populated collagen matrix40,41,42, and by including components of the vascular network43,44,45.
Although it is possible to tune the culture conditions according to specific needs, there are parameters that must be respected to guarantee both the quality and relevance of a&#160;RhE. To cultivate RhE tissues, normal human epidermal keratinocytes (NHEKs) are seeded into specific permeable culture inserts whose porous synthetic membrane separates the wells into two compartments, i.e., the apical and basolateral compartment. The porosity of the membrane (i.e., a pore size of 0.4 &#181;m) is such that it allows the formation of a cell monolayer in the apical compartment with no migration of cells to the basal insert side, and the feeding of the keratinocytes with essential nutrients from the culture medium contained in the basolateral compartment. At the beginning of the reconstitution process, NHEKs are cultured in submerged conditions for a few days to allow their adhesion onto&#160;the membrane. The calcium level in both compartments is increased compared to the calcium concentration used for the 2D culture of NHEKs to slow down the proliferation of cells and promote instead their differentiation46. An epidermal calcium gradient is essential to regulate the barrier formation and homeostasis47, 48. High calcium levels (i.e., up to 1.5 mM) promote the formation of intercellular junctions and modulate the formation of the cornified envelope during terminal differentiation49. Once keratinocytes form a continuous and tightly adherent monolayer on the supporting membrane, the medium from the apical compartment is removed and the culture process&#160;continues at the air-liquid interface (ALI) to stimulate stratification and establish an epidermal barrier50, 51. Specific culture conditions are crucial to obtain a fully stratified epithelium36. During the reconstitution process at ALI, the medium in the basolateral compartment is supplemented with keratinocyte growth factor (KGF), insulin, calcium, and ascorbic acid. Ascorbic acid plays a major role in the formation of an appropriate SC lipid barrier, closely resembling that of the native human skin52. Keratinocytes grown in ascorbic acid-supplemented medium demonstrate a differentiated phenotype, with an enhanced number of keratohyalin granules, as well as organized intercellular lipid lamellae in the interstices of the corneocytes52. Such supplementation is essential to improve epidermal barrier function by increasing cornified envelope content and avoiding depletion of hydrophilic antioxidant stores53, 54. KGF, an important paracrine mediator of epidermal proliferation and differentiation, is used to stimulate the NHEKs55.
The main downsides of in-house RhEs include the loss of standardization between research institutions and increased labor intensity and time consumption (up to 3 weeks compared to the ready-to-use commercial models). The aim of the present paper is to address these drawbacks, setting the basis for production at a larger scale. In addition to the abovementioned advantages of in-house RhEs, the current protocol aims to reduce the intra- and inter-variability among tissues, to reduce contamination risks, and to streamline the cultivation process.
The current protocol describes a reproducible and robust method to cultivate RhEs using neonatal NHEKs. Moreover, it shows representative results of the characterization of the RhEs morphology, barrier integrity, and expression of proteins that are specific for epidermal differentiation. RhEs morphological structure was examined using hematoxylin and eosin (H&#38;E) staining and transmission electron microscopy (TEM). To evaluate the barrier integrity, the transepidermal electrical resistance (TEER) and the exposure time to Triton X-100 to reduce 50% of the tissue viability (ET50) were&#160;measured. The formation of desmosomal junctions (i.e., desmoglein 1) was analyzed by immunofluorescence (IF) to evaluate keratinocyte adhesion. The formation of epidermal structural proteins (i.e., involucrin, loricrin, and filaggrin) was evaluated and detected with IF. These proteins are involved in the formation of the highly cross-linked protein envelope that surrounds SC corneocytes and as a result are important markers for late-stage epidermal differentiation56, 57. Additionally, IF was used to analyze keratin 10, a protein induced in early-stage differentiated cells in the SS58 and found inside all differentiated layers. Finally, the RhE&#39;s response to proinflammatory stimuli (i.e., lipopolysaccharide and tumor necrosis factor alpha) was investigated. The levels of interleukin 1 alpha (IL-1&#945;) and interleukin 8 (IL-8) were measured in the cell culture media, using enzyme-linked immunosorbent assays (ELISA).

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr >
			<td >Acetic acid</td>
			<td >Sigma Aldrich</td>
			<td >A6283</td>
			<td >Acidify Eosin solution with 1 mL acetic acid per 100 mL.</td>
		</tr>
		<tr >
			<td >Antibiotic-antimycotic (100x)</td>
			<td >Thermo Fisher Scientific (Gibco)</td>
			<td>15240062</td>
			<td></td>
		</tr>
		<tr >
			<td >Aqueous Eosin Y solution</td>
			<td >Sigma Aldrich</td>
			<td >HT110280</td>
			<td >Acidify Eosin solution with 1 mL acetic acid per 100 mL.</td>
		</tr>
		<tr >
			<td >Calcium chloride (CaCl2)</td>
			<td >Sigma Aldrich or Merck</td>
			<td>C8106 or 1.02378.0500</td>
			<td >Prepare a stock solution of 0.144 M, filter sterilize and store aliquots at -20 &#176;C.&#160; It is recommended to prepare new aliquots every 6 months.</td>
		</tr>
		<tr >
			<td >DAPI</td>
			<td>Roche</td>
			<td>10236276001</td>
			<td>Prepare a stock at 100 &#956;g/mL and store aliquots at -20 &#176;C. Working conditon is 1 &#956;g/mL.</td>
		</tr>
		<tr >
			<td >Entellan mounting medium</td>
			<td >Merck</td>
			<td>1.07960.0500</td>
			<td>Mounting medium for H&#38;E staining.</td>
		</tr>
		<tr >
			<td >EpiLife medium (referred to as keratinocyte growth medium)</td>
			<td >Thermo Fisher Scientific (Gibco)</td>
			<td>MEPI500CA</td>
			<td >Contains 0.06 mM of Ca2+.</td>
		</tr>
		<tr >
			<td >Ethanol absolute</td>
			<td >Any supplier</td>
			<td >N/A</td>
			<td></td>
		</tr>
		<tr >
			<td >Formalin 10%</td>
			<td >Leica Biosystems</td>
			<td >3800770</td>
			<td></td>
		</tr>
		<tr >
			<td >Human keratinocytes growth supplement (HKGS) (100x)</td>
			<td >Thermo Fisher Scientific (Gibco)</td>
			<td>S0015</td>
			<td >To reach final concentrations of 0.2% [v/v] BPE, 0.2 ng/mL human recombinant EGF, 0.18 &#956;g/mL hydrocortisone,5 &#956;g/mL bovine transferrin, and 0.01 &#181;g/mL human recombinant insulin-like growth factor-I.</td>
		</tr>
		<tr >
			<td >Isopropanol</td>
			<td >Biosolve B.V.</td>
			<td >0016264102BS</td>
			<td></td>
		</tr>
		<tr >
			<td >Kaiser&#39;s glycerol gelatine, phenol-free</td>
			<td >Merck</td>
			<td>1.08635.0100</td>
			<td>Mounting medium for IF staining.</td>
		</tr>
		<tr >
			<td >Keratinocyte growth factor (KGF)</td>
			<td >R&#38;D Systems</td>
			<td>251-KG-050</td>
			<td >Reconstitute KGF protein at 100 &#956;g/mL in sterile 0.1% [w/v] BSA in PBS. Prepare aliquots and store at -20&#176;C.&#160;</td>
		</tr>
		<tr >
			<td >Lactate dehydrogenase (LDH) assay kit</td>
			<td >Roche</td>
			<td >4744926001</td>
			<td></td>
		</tr>
		<tr >
			<td >L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate&#160;</td>
			<td >Sigma-Aldrich</td>
			<td>A8960</td>
			<td >Prepare a stock solution of 25 mg/mL, filter sterilize and store aliquots at -20&#176;C. It is recommended to prepare new aliquots every 6 months. Store in the dark.</td>
		</tr>
		<tr >
			<td >Lipopolysaccharide (LPS), E.coli strain O55:B5</td>
			<td >Sigma-Aldrich</td>
			<td >L4524</td>
			<td >Prepare a stock of 1 mg/mL in sterile PBS and store aliquots at -20 &#176;C. Working concentration is 100 &#956;g/mL.&#160;</td>
		</tr>
		<tr >
			<td >Mayer&#8217;s Haematoxylin</td>
			<td >Sigma-Aldrich</td>
			<td >MHS80</td>
			<td></td>
		</tr>
		<tr >
			<td >Normal human epidermal keratinocytes (NHEKs)</td>
			<td >Lonza</td>
			<td>00192906</td>
			<td >Human primary keratinocytes isolated from neonatal foreskin, pooled from at least three donors.</td>
		</tr>
		<tr >
			<td >Phosphate-buffered saline</td>
			<td >Thermo Fisher Scientific (Gibco)</td>
			<td>10010056 or 10010-015</td>
			<td >Reference numbers can vary between countries.</td>
		</tr>
		<tr >
			<td >Recombinant tumor necrosis factor alpha (TNF-&#945;)</td>
			<td >ImmunoTools</td>
			<td >11344483</td>
			<td>Prepare a stock of 100 &#956;g/mL in sterile ultrapure water. Working concentration is 40 ng/mL</td>
		</tr>
		<tr >
			<td >Sterile ultrapure water</td>
			<td >Any supplier</td>
			<td >N/A</td>
			<td></td>
		</tr>
		<tr >
			<td >Thiazolyl Blue Tetrazolium Bromide (MTT)</td>
			<td >Sigma-Aldrich</td>
			<td >M5655</td>
			<td>Prepare a stock of 5 mg/mL MTT in sterile PBS. Working concentration is 1 mg/mL.</td>
		</tr>
		<tr >
			<td >Triton X-100</td>
			<td >Sigma-Aldrich</td>
			<td>93426</td>
			<td></td>
		</tr>
		<tr >
			<td >Trypan blue (0.4% [v/v])</td>
			<td >Any supplier</td>
			<td >N/A</td>
			<td></td>
		</tr>
		<tr >
			<td >Trypsin inhibitor</td>
			<td >Thermo Fisher Scientific (Gibco)</td>
			<td>R007100</td>
			<td></td>
		</tr>
		<tr >
			<td >Trypsin/EDTA (0.05% [v/v])</td>
			<td >Thermo Fisher Scientific (Gibco)</td>
			<td>25300054</td>
			<td></td>
		</tr>
		<tr >
			<td >Tween 20</td>
			<td >Sigma-Aldrich</td>
			<td >P1379</td>
			<td></td>
		</tr>
		<tr >
			<td >Xylene</td>
			<td >Sigma-Aldrich</td>
			<td>214736</td>
			<td></td>
		</tr>
		<tr >
			<td >Goat Anti-Mouse IgG H&#38;L (Alexa Fluor 488)</td>
			<td >Abcam</td>
			<td>Ab150113</td>
			<td></td>
		</tr>
		<tr >
			<td >Tri-sodium citrate dihydrate</td>
			<td >Merck</td>
			<td>1.06448.0500</td>
			<td>For antigen retrieval buffer for IF staining.&#160;</td>
		</tr>
		<tr >
			<td >Goat Anti-Rabbit IgG H&#38;L (Dylight 488)</td>
			<td >Agrisera</td>
			<td>AS09 633</td>
			<td></td>
		</tr>
		<tr >
			<td >Filaggrin Mouse antibody</td>
			<td >BioTechne (Novus Biologicals)</td>
			<td>NBP2-53243</td>
			<td></td>
		</tr>
		<tr >
			<td >Involucrin Rabbit antibody</td>
			<td >BioTechne (Novus Biologicals)</td>
			<td>NBP2-33742</td>
			<td></td>
		</tr>
		<tr >
			<td >Keratin 10 Mouse antibody</td>
			<td >BioTechne (Novus Biologicals)</td>
			<td>NBP2-32962</td>
			<td></td>
		</tr>
		<tr >
			<td >Desmoglein-1 Mouse antibody&#160;</td>
			<td >BioTechne (Novus Biologicals)</td>
			<td>MAB944</td>
			<td></td>
		</tr>
		<tr >
			<td >Loricrin Rabbit antibody</td>
			<td >BioTechne (Novus Biologicals)</td>
			<td>NBP1-33610</td>
			<td></td>
		</tr>
	</tbody>
</table>

cultivating a three-dimensional reconstructed human epidermis at a large scale

A three-dimensional human epidermis model reconstructed from neonatal primary keratinocytes is presented. Herein, a protocol for the cultivation process and the characterization of the model is described. Neonatal primary keratinocytes are grown submerged on permeable polycarbonate inserts and lifted to the air-liquid interface three days after seeding. After fourteen days of stimulation with defined growth factors and ascorbic acid in high calcium culture medium, the model is fully differentiated. Histological analysis revealed a completely stratified epidermis, mimicking the morphology of native human skin. To characterize the model and its barrier functions, protein levels and localization specific for early-stage keratinocyte differentiation (i.e., keratin 10), late-stage differentiation (i.e., involucrin, loricrin, and filaggrin) and tissue adhesion (i.e., desmoglein 1), were assessed by immunofluorescence. The tissue barrier integrity was further evaluated by measuring transepithelial electrical resistance. Reconstructed human epidermis was responsive to proinflammatory stimuli (i.e., lipopolysaccharide and tumor necrosis factor alpha), leading to increased cytokine release (i.e., interleukin 1 alpha and interleukin 8). This protocol represents a straightforward and reproducible in vitro method to cultivate reconstructed human epidermis as a tool to assess environmental effects and a broad range of skin-related studies.

NHEKs cultured in 2D display a traditional morphology with a consistent polygonal shape (Figure 2A).&#160;As described above, NHEKs are seeded into culture inserts after reaching a confluency of approximately 80%. The morphology of the RhEs was analyzed using&#160;H&#38;E&#160;staining and TEM. After 15 days at ALI,&#160;a fully stratified tissue is obtained as indicated by its four main epidermal layers: the SB, the SS, the SG, and the SC (Figure 2B). In the SB layer, the cells have a columnar shape. From the second layer on towards the upper layers of the RhE,&#160;NHEKs differentiate as observed by the changes in the cell morphology (from&#160;a columnar shape in the SB layer, towards a spinous shape in the SS layer). In the SG layer, the cells have a more flattened shape and display keratohyalin granules (KG) that are represented as purple dots in the cytoplasm. Their characteristic round and stellar shape is&#160;highlighted by white arrows on the H&#38;E image (Figure 2C). The cells in the SC, are terminally differentiated and are completely flattened and lack a cell nucleus. The stratified RhEs have an overall thickness of 84.3 &#177; 2.4 &#181;m and their SC has a thickness of 19.6 &#177; 3.2 &#181;m (Figure 2D). These values are comparable to those reported for native human skin, i.e., 60-120 &#181;m and 10-20 &#181;m, respectively69. The number of viable layers is 6-7, which is lower compared to that of native human skin, being approximately 7-1470. Ultrastructural analysis of RhEs at different time points in the reconstitution protocol (i.e., 7, 10, 13, and 15 days) reveals the cornification process of the RhEs with an increased number of corneocyte layers over time (Figure 2E). After 15 days at the ALI, the SC of the RhE tissue is made of approximately 15-25 layers, which is comparable to the value reported for native human skin (i.e., 15-20 layers)69.
<img alt="Figure 2" class="xfigimg" src="/files/ftp_upload/61802/61802fig2v2.jpg" /> 
Figure 2: Primary keratinocytes and&#160;reconstructed human epidermis.&#160;(A) Phase-contrast microscopy image of primary keratinocytes before seeding onto inserts. Scale bar is 50 &#181;m. (B-C)&#160;H&#38;E bright field microscopy image of RhE. Scale bar is 50 &#181;m (B) and 25 &#181;m (C). (D)&#160;Quantification of thickness of RhE and SC (mean &#177; SEM, n=3). (E)&#160;Transmission electron microscopy images of RhE cross-sections after 7, 10, 13, and 15 days at ALI. Scale bar is 4 &#181;m.&#160;<a href="https://www.jove.com/files/ftp_upload/61802/61802fig2v2large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
According to their differentiation stage,&#160;NHEKs growing in 3D show different protein expression profiles according to their differentiation stage. The expression of proteins specific for early-stage keratinocyte differentiation (i.e., keratin 10), late-stage keratinocyte differentiation (i.e., involucrin, loricrin, and filaggrin), and keratinocyte adhesion (i.e., desmoglein 1) in RhEs was determined using&#160;IF&#160;staining. Involucrin expression appears more predominantly located in the SG layer since its expression is initiated earlier during the differentiation process (Figure 3D), whereas&#160;filaggrin and loricrin are expressed&#160;in the upper layers (Figure 3B-C). Keratin 10 expression was found in all the viable layers, except of the SB layer (Figure 3E). RhEs display functional desmosomal junctions, as indicated by the expression of desmoglein 1 in the intercellular space of the viable epidermal layers (Figure 3F). To conclude, all five markers are expressed and located in the appropriate epidermal layers and translate to a healthy epidermal differentiation process.
<img alt="Figure 3" class="xfigimg" src="/files/ftp_upload/61802/61802fig03.jpg" /> 
Figure 3: Epidermal differentiation, tissue adhesion, and tissue integrity of reconstructed human epidermis.&#160;(A)&#160;H&#38;E bright field microscopy image of RhE. Confocal fluorescence microscopy images of (B) filaggrin (FLG), (C) loricrin (LOR), (D)&#160;involucrin (INV),&#160;(E) keratin 10 (K10), and&#160;(F)&#160;desmoglein 1 (DSG-1) represented in magenta. Nuclei staining (DAPI) is represented in blue. Scale bar is 25 &#181;m.&#160;<a href="https://www.jove.com/files/ftp_upload/61802/61802fig03large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
The barrier properties of the RhE model was investigated by assessing both the tissue viability&#160;and&#160;integrity. The tissue integrity was determined after 15 days by measuring the TEER using a voltohmmeter. The 2567 &#177; 415 &#937;.cm2 values recorded for the RhEs translate the formation of a continuous barrier (Figure 4A). Those values are in range with those reported for RhE models71,72,73,74. Additionally, the required exposure time for a cytotoxic reference chemical (i.e., Triton X-100) to reduce the tissue viability by 50% (ET50) was determined with a thiazolyl blue tetrazolium bromide (MTT) assay. The ET50 value measured for the RhE was 2.1 hours. This value falls within the acceptance range of other 3D epidermal models that are qualified for reliable prediction of irritation classification (OECD Guideline 439)19.
<img alt="Figure 4" class="xfigimg" src="/files/ftp_upload/61802/61802fig4v3.jpg" /> 
Figure 4: Barrier properties of reconstructed human epidermis.&#160;(A) Tissue integrity measured with transepithelial electrical resistance (mean &#177; SEM, n=6). (B) ET50&#160;determined by measuring tissue viability (i.e., MTT assay) upon topical exposure to 78.3 &#181;L of 1% Triton X-100 (mean &#177; SEM, n=3).&#160;
Responsiveness of RhEs was investigated upon known proinflammatory stimuli. RhEs were treated systemically, i.e., addition of stimuli in the medium of the basolateral compartment, using 100 &#181;g/mL Escherichia coli lipopolysaccharide (LPS) and 40 ng/mL tumor necrosis factor alpha (TNF-&#945;). After 24 hours of stimuli, the cell culture medium was collected. The cytotoxicity was measured using a lactate dehydrogenase (LDH) assay and compared to values of a known membrane disruptor, the Triton X-100 detergent (Figure 5). A significant increase (p &#60;&#160;0.05, one-way ANOVA, Dunnett&#39;s multiple comparison test) was shown in LDH activity&#160;in RhEs treated with Triton X-100. LPS and TNF-&#945; treatments both showed not be cytotoxic.
<img alt="Figure 5" class="xfigimg" src="/files/ftp_upload/61802/61802fig5v3.jpg" /> 
Figure 5: The cytotoxicity measured via lactate dehydrogenase (LDH) assay. Data are&#160;presented as relative values to control, untreated tissues (CTRL); mean &#177; SEM, n=9 (Triton X-100), n=8 (LPS), n=3 (TNF-&#945;). Significance was tested with one-way ANOVA, Dunnett&#39;s multiple comparison test. Asterisk denotes statistically significant difference compared to CTRL, ****p &#60; 0.0001).&#160;<a href="https://www.jove.com/files/ftp_upload/61802/61802fig5v3large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
The release of interleukin 1 alpha (IL-1&#945;) and interleukin 8 (IL-8) in the RhE medium was quantified using ELISAs. Figure 6 shows both the quantified and relative IL-1&#945; and IL-8 release by the RhEs upon challenge with LPS and TNF-&#945;. LPS treatment resulted in a statistically significant (p &#60;&#160;0.05, unpaired Student&#39;s T-test) induced&#160;release of IL-8 (9.6 &#177; 1.0 fold increase) and IL-1&#945; (2.7 &#177; 1.3 fold increase). TNF-&#945; did not significantly induce&#160;IL-8&#160;release, even though a tendency of increased IL-8 levels was observed (2.3 &#177; 0.8 fold increase).&#160;However, TNF-&#945; did significantly&#160;(p &#60;&#160;0.05, unpaired Student&#39;s T-test) trigger IL-1&#945; release (1.8 &#177; 0.5 fold increase).
<img alt="Figure 6" class="xfigimg" src="/files/ftp_upload/61802/61802fig6v3.jpg" /> 
Figure 6: Proinflammatory responses in the reconstructed human epidermis.&#160;Concentrations of IL-8 release by&#160;the RhE upon a 24-hour challenge with LPS (A) and TNF-&#945; (B).&#160;Data is represented as mean &#177; SEM, n=8 (LPS), n=3 (TNF-&#945;). (C)&#160;Data is represented as the mean of relative value compared to control, untreated tissues (CTRL) &#177; SEM, n=8 (LPS), n=3 (TNF-&#945;). The concentration of IL-1&#945; release of the RhE upon a 24-hour challenge with LPS (D) and TNF-&#945; (E).&#160;Data is represented as mean &#177; SEM, n=8 (LPS), n=3 (TNF-&#945;). (F)&#160;Data is represented as the mean of relative value compared to CTRL &#177; SEM, n=4 (LPS), n=3 (TNF-&#945;). Significance was tested by an unpaired Student&#39;s T-test. Asterisk denotes statistically significant differences&#160;compared to CTRL, *p &#60; 0.05, ****p &#60; 0.0001).&#160;<a href="https://www.jove.com/files/ftp_upload/61802/61802fig6v3large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>

Watch this Scientific Journal Video about Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale at JoVE.com

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

This protocol describes a straightforward method to cultivate three-dimensional reconstituted human epidermis in a reproducible and robust manner. Additionally, it characterizes the structure-function relationship of the epidermal barrier model. The biological responses of the reconstituted human epidermis upon proinflammatory stimuli are also presented.

A three-dimensional human epidermis model reconstructed from neonatal primary keratinocytes is presented. Herein, a protocol for the cultivation process ...

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10.4K Views.  University of Fribourg. This protocol describes a straightforward method to cultivate three-dimensional reconstituted human epidermis in a reproducible and robust manner. Additionally, it characterizes the structure-function relationship of the epidermal barrier model. The biological responses of the reconstituted human epidermis upon proinflammatory stimuli are also presented.

Video: Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Alginate Hydrogels for Three-Dimensional Organ Culture of Ovaries and Oviducts

Ovarian cancer is the fifth leading cause of cancer deaths in women and has a 63% mortality rate in the United States1. The cell type of origin for ovarian cancers is still in question and might be either the ovarian surface epithelium (OSE) or the distal epithelium of the fallopian tube fimbriae2,3. Culturing the normal cells as a primary culture in vitro will enable scientists to model specific changes that might lead to ovarian cancer in the distinct epithelium, thereby definitively determining the cell type of origin. This will allow development of more accurate biomarkers, animal models with tissue-specific gene changes, and better prevention strategies targeted to this disease.
Maintaining normal cells in alginate hydrogels promotes short term in vitro culture of cells in their three-dimensional context and permits introduction of plasmid DNA, siRNA, and small molecules. By culturing organs in pieces that are derived from strategic cuts using a scalpel, several cultures from a single organ can be generated, increasing the number of experiments from a single animal. These cuts model aspects of ovulation leading to proliferation of the OSE, which is associated with ovarian cancer formation. Cell types such as the OSE that do not grow well on plastic surfaces can be cultured using this method and facilitate investigation into normal cellular processes or the earliest events in cancer formation4.
Alginate hydrogels can be used to support the growth of many types of tissues5. Alginate is a linear polysaccharide composed of repeating units of &beta;-D-mannuronic acid and &alpha;-L-guluronic acid that can be crosslinked with calcium ions, resulting in a gentle gelling action that does not damage tissues6,7. Like other three-dimensional cell culture matrices such as Matrigel, alginate provides mechanical support for tissues; however, proteins are not reactive with the alginate matrix, and therefore alginate functions as a synthetic extracellular matrix that does not initiate cell signaling5. The alginate hydrogel floats in standard cell culture medium and supports the architecture of the tissue growth in vitro.
A method is presented for the preparation, separation, and embedding of ovarian and oviductal organ pieces into alginate hydrogels, which can be maintained in culture for up to two weeks. The enzymatic release of cells for analysis of proteins and RNA samples from the organ culture is also described. Finally, the growth of primary cell types is possible without genetic immortalization from mice and permits investigators to use knockout and transgenic mice.

Culture of normal cells in their three-dimensional context represents an alternative method to study early events required for cellular transformation and tumorigenesis. This method is used to grow normal ovarian and oviductal cells to study early events in ovarian cancer formation.

Ovarian cancer is the fifth leading cause of cancer deaths in women and has a 63% mortality rate in the United States1. The cell type of origin for ...

The Three-Dimensional Human Skin Reconstruct Model: a Tool to Study Normal  Skin and Melanoma Progression

Most in vitro studies in experimental skin biology have been done in 2-dimensional (2D) monocultures, while accumulating evidence suggests that cells behave differently when they are grown within a 3D extra-cellular matrix and also interact with other cells (1-5). Mouse models have been broadly utilized to study tissue morphogenesis in vivo. However mouse and human skin have significant differences in cellular architecture and physiology, which makes it difficult to extrapolate mouse studies to humans. Since melanocytes in mouse skin are mostly localized in hair follicles, they have distinct biological properties from those of humans, which locate primarily at the basal layer of the epidermis. The recent development of 3D human skin reconstruct models has enabled the field to investigate cell-matrix and cell-cell interactions between different cell types. The reconstructs consist of a "dermis" with fibroblasts embedded in a collagen I matrix, an "epidermis", which is comprised of stratified, differentiated keratinocytes and a functional basement membrane, which separates epidermis from dermis. Collagen provides scaffolding, nutrient delivery, and potential for cell-to-cell interaction. The 3D skin models incorporating melanocytic cells recapitulate natural features of melanocyte homeostasis and melanoma progression in human skin. As in vivo, melanocytes in reconstructed skin are localized at the basement membrane interspersed with basal layer keratinocytes. Melanoma cells exhibit the same characteristics reflecting the original tumor stage (RGP, VGP and metastatic melanoma cells) in vivo. Recently, dermal stem cells have been identified in the human dermis (6). These multi-potent stem cells can migrate to the epidermis and differentiate to melanocytes.

In this report, we describe the three-dimensional skin reconstruct model which mimics human skin in architecture and composition. Melanocyte physiology, melanoma progression and the fate of dermal stem cells have been investigated using the skin reconstruct model. The model is also useful as a preclinical tool for drug assessment.

Most in vitro studies in experimental skin biology have been done in 2-dimensional (2D) monocultures, while accumulating evidence suggests that cells ...

Planar and Three-Dimensional Printing of Conductive Inks

Printed electronics rely on low-cost, large-area fabrication routes to create flexible or multidimensional electronic, optoelectronic, and biomedical devices1-3. In this paper, we focus on one- (1D), two- (2D), and three-dimensional (3D) printing of conductive metallic inks in the form of flexible, stretchable, and spanning microelectrodes.
Direct-write assembly4,5 is a 1-to-3D printing technique that enables the fabrication of features ranging from simple lines to complex structures by the deposition of concentrated inks through fine nozzles (~0.1 - 250 &mu;m). This printing method consists of a computer-controlled 3-axis translation stage, an ink reservoir and nozzle, and 10x telescopic lens for visualization. Unlike inkjet printing, a droplet-based process, direct-write assembly involves the extrusion of ink filaments either in- or out-of-plane. The printed filaments typically conform to the nozzle size. Hence, microscale features (&lt; 1 &mu;m) can be patterned and assembled into larger arrays and multidimensional architectures.
In this paper, we first synthesize a highly concentrated silver nanoparticle ink for planar and 3D printing via direct-write assembly. Next, a standard protocol for printing microelectrodes in multidimensional motifs is demonstrated. Finally, applications of printed microelectrodes for electrically small antennas, solar cells, and light-emitting diodes are highlighted.

Planar and three-dimensional printing of conductive metallic inks is described. Our approach provides new avenues for fabricating printed electronic, optoelectronic, and biomedical devices in unusual layouts at the microscale.

Printed electronics rely on low-cost, large-area fabrication routes to create flexible or multidimensional electronic, optoelectronic, and biomedical ...

Investigating the Three-dimensional Flow Separation Induced by a Model Vocal Fold Polyp

The fluid-structure energy exchange process for normal speech has been studied extensively, but it is not well understood for pathological conditions. Polyps and nodules, which are geometric abnormalities that form on the medial surface of the vocal folds, can disrupt vocal fold dynamics and thus can have devastating consequences on a patient&#39;s ability to communicate. Our laboratory has reported particle image velocimetry (PIV) measurements, within an investigation of a model polyp located on the medial surface of an in&#160;vitro driven vocal fold model, which show that such a geometric abnormality considerably disrupts the glottal jet behavior. This flow field adjustment is a likely reason for the severe degradation of the vocal quality in patients with polyps. A more complete understanding of the formation and propagation of vortical structures from a geometric protuberance, such as a vocal fold polyp, and the resulting influence on the aerodynamic loadings that drive the vocal fold dynamics, is necessary for advancing the treatment of this pathological condition. The present investigation concerns the three-dimensional flow separation induced by a wall-mounted prolate hemispheroid with a 2:1 aspect ratio in cross flow, i.e. a model vocal fold polyp, using an oil-film visualization technique. Unsteady, three-dimensional flow separation and its impact of the wall pressure loading are examined using skin friction line visualization and wall pressure measurements.

Vocal fold polyps can disrupt vocal fold dynamics and thus can have devastating consequences on a patient&#39;s ability to communicate. Three-dimensional flow separation induced by a wall-mounted model polyp and its impact on the wall pressure loading are examined using particle image velocimetry, skin friction line visualization, and wall pressure measurements.

The fluid-structure energy exchange process for normal speech has been studied extensively, but it is not well understood for pathological conditions. ...

Human Cartilage Tissue Fabrication Using Three-dimensional Inkjet Printing Technology

Bioprinting, which is based on thermal inkjet printing, is one of the most attractive enabling technologies in the field of tissue engineering and regenerative medicine. With digital control&#160;cells, scaffolds, and growth factors can be precisely deposited to the desired two-dimensional (2D) and three-dimensional (3D) locations rapidly. Therefore, this technology is an ideal approach to fabricate tissues mimicking their native anatomic structures. In order to engineer cartilage with native zonal organization, extracellular matrix composition (ECM), and mechanical properties, we developed a bioprinting platform using a commercial inkjet printer with simultaneous photopolymerization capable for 3D cartilage tissue engineering. Human chondrocytes suspended in poly(ethylene glycol) diacrylate (PEGDA) were printed for 3D neocartilage construction via layer-by-layer assembly. The printed cells were fixed at their original deposited positions, supported by the surrounding scaffold in simultaneous photopolymerization. The mechanical properties of the printed tissue were similar to the native cartilage. Compared to conventional tissue fabrication, which requires longer UV exposure, the viability of the printed cells with simultaneous photopolymerization was significantly higher. Printed neocartilage demonstrated excellent glycosaminoglycan (GAG) and collagen type II production, which was consistent with gene expression. Therefore, this platform is ideal for accurate cell distribution and arrangement for anatomic tissue engineering.

The methods described in this paper show how to convert a commercial inkjet printer into a bioprinter with simultaneous UV polymerization. The printer is capable of constructing 3D tissue structure with cells and biomaterials. The study demonstrated here constructed a 3D neocartilage.

Bioprinting, which is based on thermal inkjet printing, is one of the most attractive enabling technologies in the field of tissue engineering and ...

Analysis of Cell Migration within a Three-dimensional Collagen Matrix

The ability to migrate is a hallmark of various cell types and plays a crucial role in several physiological processes, including&#160;embryonic development, wound healing, and immune responses. However, cell migration is also a key mechanism in cancer enabling these cancer cells to detach from the primary tumor to start metastatic spreading. Within the past years various cell migration assays have been developed to analyze the migratory behavior of different cell types. Because the locomotory behavior of cells markedly differs between a two-dimensional (2D) and three-dimensional (3D) environment it can be assumed that the analysis of the migration of cells that are embedded within a 3D environment would yield in more significant cell migration data. The advantage of the described 3D collagen matrix migration assay is that cells are embedded within a physiological 3D network of collagen fibers representing the major component of the extracellular matrix. Due to time-lapse video microscopy real cell migration is measured allowing the determination of several migration parameters as well as their alterations in response to pro-migratory factors or inhibitors. Various cell types could be analyzed using this technique, including lymphocytes/leukocytes, stem cells, and tumor cells. Likewise, also cell clusters or spheroids could be embedded within the collagen matrix concomitant with analysis of the emigration of single cells from the cell cluster/ spheroid into the collagen lattice. We conclude that the 3D collagen matrix migration assay is a versatile method to analyze the migration of cells within a physiological-like 3D environment.

Cell migration is a biological phenomenon that is involved in a plethora of physiological, such as wound healing and immune responses, and pathophysiological processes, like cancer. The 3D-collagen matrix migration assay is a versatile tool to analyze the migratory properties of different cell types within in a 3D physiological-like environment.

The ability to migrate is a hallmark of various cell types and plays a crucial role in several physiological processes, including&#160;embryonic ...

Cloning and Large-Scale Production of High-Capacity Adenoviral Vectors Based on the Human Adenovirus Type 5

High-capacity adenoviral vectors (HCAdV) devoid of all viral coding sequences represent one of the most advanced gene delivery vectors due to their high packaging capacity (up to 35 kb), low immunogenicity, and low toxicity. However, for many laboratories the use of HCAdV is hampered by the complicated procedure for vector genome construction and virus production. Here, a detailed protocol for efficient cloning and production of HCAdV based on the plasmid pAdFTC containing the HCAdV genome is described. The construction of HCAdV genomes is based on a cloning vector system utilizing homing endonucleases (I-CeuI and PI-SceI). Any gene of interest of up to 14 kb can be subcloned into the shuttle vector pHM5, which contains a multiple cloning site flanked by I-CeuI and PI-SceI. After I-CeuI and PI-SceI-mediated release of the transgene from the shuttle vector the transgene can be inserted into the HCAdV cloning vector pAdFTC. Because of the large size of the pAdFTC plasmid and the long recognition sites of the used enzymes associated with strong DNA binding, careful handling of the cloning fragments is needed. For virus production, the HCAdV genome is released by NotI digest and transfected into a HEK293 based producer cell line stably expressing Cre recombinase. To provide all adenoviral genes for adenovirus amplification, co-infection with a helper virus containing a packing signal flanked by loxP sites is required. Pre-amplification of the vector is performed in producer cells grown on surfaces and large-scale amplification of the vector is conducted in spinner flasks with producer cells grown in suspension. For virus purification, two ultracentrifugation steps based on cesium chloride gradients are performed followed by dialysis. Here tips, tricks, and shortcuts developed over the past years working with this HCAdV vector system are presented.

A protocol for generation of high-capacity adenoviral vectors lacking all viral coding sequences is presented. Cloning of transgenes contained in the vector genome is based on homing endonucleases. Virus amplification in producer cells grown as adherent cells and in suspension relies on a helper virus providing viral genes in trans.

High-capacity adenoviral vectors (HCAdV) devoid of all viral coding sequences represent one of the most advanced gene delivery vectors due to their high ...

Three-dimensional Biomimetic Technology: Novel Biorubber Creates Defined Micro- and Macro-scale Architectures in Collagen Hydrogels

Tissue scaffolds play a crucial role in the tissue regeneration process. The ideal scaffold must fulfill several requirements such as having proper composition, targeted modulus, and well-defined architectural features. Biomaterials that recapitulate the intrinsic architecture of in vivo tissue are vital for studying diseases as well as to facilitate the regeneration of lost and malformed soft tissue. A novel biofabrication technique was developed which combines state of the art imaging, three-dimensional (3D) printing, and selective enzymatic activity to create a new generation of biomaterials for research and clinical application. The developed material, Bovine Serum Albumin rubber, is reaction injected into a mold that upholds specific geometrical features. This sacrificial material allows the adequate transfer of architectural features to a natural scaffold material. The prototype consists of a 3D collagen scaffold with 4 and 3 mm channels that represent a branched architecture. This paper emphasizes the use of this biofabrication technique for the generation of natural constructs. This protocol utilizes a computer-aided software (CAD) to manufacture a solid mold which will be reaction injected with BSA rubber followed by the enzymatic digestion of the rubber, leaving its architectural features within the scaffold material.

An innovative biofabrication technique was developed to engineer three-dimensional constructs that resemble the architectural features, components, and mechanical properties of in vivo tissue. This technique features a newly developed sacrificial material, BSA rubber, which transfers detailed spatial features, reproducing the in vivo architectures of a wide variety of tissues.

Tissue scaffolds play a crucial role in the tissue regeneration process. The ideal scaffold must fulfill several requirements such as having proper ...

Preparation and Analysis of In Vitro Three Dimensional Breast Carcinoma Surrogates

Three dimensional (3D) culture is a more physiologically relevant method to model cell behavior in vitro than two dimensional culture. Carcinomas, including breast carcinomas, are complex 3D tissues composed of cancer epithelial cells and stromal components, including fibroblasts and extracellular matrix (ECM). Yet most in vitro models of breast carcinoma consist only of cancer epithelial cells, omitting the stroma and, therefore, the 3D architecture of a tumor in vivo. Appropriate 3D modeling of carcinoma is important for accurate understanding of tumor biology, behavior, and response to therapy. However, the duration of culture and volume of 3D models is limited by the availability of oxygen and nutrients within the culture. Herein, we demonstrate a method in which breast carcinoma epithelial cells and stromal fibroblasts are incorporated into ECM to generate a 3D breast cancer surrogate that includes stroma and can be cultured as a solid 3D structure or by using a perfusion bioreactor system to deliver oxygen and nutrients. Following setup and an initial growth period, surrogates can be used for preclinical drug testing. Alternatively, the cellular and matrix components of the surrogate can be modified to address a variety of biological questions. After culture, surrogates are fixed and processed to paraffin, in a manner similar to the handling of clinical breast carcinoma specimens, for evaluation of parameters of interest. The evaluation of one such parameter, the density of cells present, is explained, where ImageJ and CellProfiler image analysis software systems are applied to photomicrographs of histologic sections of surrogates to quantify the number of nucleated cells per area. This can be used as an indicator of the change in cell number over time or the change in cell number resulting from varying growth conditions and treatments.

Preparation and Analysis of In Vitro Three Dimensional Breast Carcinoma Surrogates

We demonstrate a method to generate 3D breast cancer surrogates, which can be cultured using a perfusion bioreactor system to deliver oxygen and nutrients. Following growth, surrogates are fixed and processed to paraffin for evaluation of parameters of interest. The evaluation of one such parameter, cell density, is explained.

Three dimensional (3D) culture is a more physiologically relevant method to model cell behavior in vitro than two dimensional culture. Carcinomas, ...

Development of a Multicellular Three-dimensional Organotypic Model of the Human Intestinal Mucosa Grown Under Microgravity

Because cells growing in a three-dimensional (3-D) environment have the potential to bridge many gaps of cell cultivation in 2-D environments (e.g., flasks or dishes). In fact, it is widely recognized that cells grown in flasks or dishes tend to de-differentiate and lose specialized features of the tissues from which they were derived. Currently, there are mainly two types of 3-D culture systems where the cells are seeded into scaffolds mimicking the native extracellular matrix (ECM): (a) static models and (b) models using bioreactors. The first breakthrough was the static 3-D models. 3-D models using bioreactors such as the rotating-wall-vessel (RWV) bioreactors are a more recent development. The original concept of the RWV bioreactors was developed at NASA&#39;s Johnson Space Center in the early 1990s and is believed to overcome the limitations of static models such as the development of hypoxic, necrotic cores. The RWV bioreactors might circumvent this problem by providing fluid dynamics that allow the efficient diffusion of nutrients and oxygen. These bioreactors consist of a rotator base that serves to support and rotate two different formats of culture vessels that differ by their aeration source type: (1) Slow Turning Lateral Vessels (STLVs) with a co-axial oxygenator in the center, or (2) High Aspect Ratio Vessels (HARVs) with oxygenation via a flat, silicone rubber gas transfer membrane. These vessels allow efficient gas transfer while avoiding bubble formation and consequent turbulence. These conditions result in laminar flow and minimal shear force that models reduced gravity (microgravity) inside the culture vessel. Here we describe the development of a multicellular 3-D organotypic model of the human intestinal mucosa composed of an intestinal epithelial cell line and primary human lymphocytes, endothelial cells and fibroblasts cultured under microgravity provided by the RWV bioreactor.

Cells growing in a three-dimensional (3-D) environment represent a marked improvement over cell cultivation in 2-D environments (e.g., flasks or dishes). Here we describe the development of a multicellular 3-D organotypic model of the human intestinal mucosa cultured under microgravity provided by rotating-wall-vessel (RWV) bioreactors.

Because cells growing in a three-dimensional (3-D) environment have the potential to bridge many gaps of cell cultivation in 2-D environments (e.g., ...