Polarization of M1 and M2 Human Monocyte-Derived Cells and Analysis with Flow Cytometry upon Mycobacterium tuberculosis Infection
28.4K Views
•
10:43 min
•
September 18th, 2020
DOI :
September 18th, 2020
•Akhirunnesa Mily1,2, Sadaf Kalsum1, Marco Giulio Loreti1, Rokeya Sultana Rekha3, Jagadeeswara Rao Muvva1, Magda Lourda1,4, Susanna Brighenti1
1Center for Infectious Medicine (CIM), Department of Medicine Huddinge, ANA Futura, Karolinska Institutet, 2Infectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh, 3Clinical Microbiology, Department of Laboratory Medicine (Labmed), ANA Futura, Karolinska Institutet, 4Childhood Cancer Research Unit, Department of Women's and Children's Health, Karolinska Institutet
Chapters in this video
0:04
Introduction
0:44
Peripheral Blood Mononuclear Cell (PBMC) Isolation
1:40
Monocyte-Derived Cell Differentiation and Polarization
3:25
Mycobacterium tuberculosis (Mtb) Culture
5:00
Monocyte-Derived Cell Mtb Infection
6:03
Flow Cytometry Staining and Analysis
7:40
Results: Representative Phenotypic M1 and M2 Cell Characterization Before and After Mtb Infection
9:47
Conclusion
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