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Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications

DOI :

10.3791/61825-v

8:54 min

November 5th, 2020

November 5th, 2020

11,319 Views

1Columbia Environmental Research Center, U.S. Geological Survey

Environmental DNA assays require rigorous design, testing, optimization and validation before the collection of field data can begin. Here, we present a protocol to take users through each step of designing a species-specific, probe-based qPCR assay for the detection and quantification of a target species DNA from environmental samples.

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Species specific Quantitative PCR Assays

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