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In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes

DOI :

10.3791/62016-v

11:42 min

June 10th, 2021

June 10th, 2021

4,192 Views

1Center for Gene Therapy, The Research Institute at Nationwide Children’s Hospital, 2College of Medicine, The Ohio State University

We describe a protocol to reprogram human skin-derived fibroblasts into induced Neuronal Progenitor Cells (iNPCs), and their subsequent differentiation into induced Astrocytes (iAs). This method leads to fast and reproducible generation of iNPCs and iAs in large quantities.

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In Vitro Modeling

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