JoVE Logo
Faculty Resource Center

Sign In

Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells

DOI :

10.3791/52831-v

July 29th, 2015

July 29th, 2015

15,152 Views

1Institute of Anatomy and Cell Biology, University of Würzburg, 2Institute of Reconstructive Neurobiology, University of Bonn, 3German Cancer Research Center, Heidelberg

Generation of induced pluripotent stem cells provides fascinating prospects for the derivation of autologous transplants. However, progression through a pluripotent state and laborious re-differentiation still hinders clinical translation. Here we describe the derivation of adult human fibroblasts and their direct conversion into induced neural progenitor cells and the subsequent differentiation into neural lineages.

Tags

Adult Human Fibroblasts

-- Views

Related Videos

article

Time-lapse Live Imaging of Clonally Related Neural Progenitor Cells in the Developing Zebrafish Forebrain

article

Neural-Colony Forming Cell Assay: An Assay To Discriminate Bona Fide Neural Stem Cells from Neural Progenitor Cells

article

Live-cell Imaging of Sensory Organ Precursor Cells in Intact Drosophila Pupae

article

Lineage-reprogramming of Pericyte-derived Cells of the Adult Human Brain into Induced Neurons

article

Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells

article

Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics

article

Development of a Refined Protocol for Trans-scleral Subretinal Transplantation of Human Retinal Pigment Epithelial Cells into Rat Eyes

article

Homochronic Transplantation of Interneuron Precursors into Early Postnatal Mouse Brains

article

Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan

article

Conversion of Human Induced Pluripotent Stem Cells (iPSCs) into Functional Spinal and Cranial Motor Neurons Using PiggyBac Vectors

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved