Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity

3.6K Views

10:03 min

January 30th, 2021

DOI :

10.3791/62182-v

January 30th, 2021


Transcript

Explore More Videos

Epidermal Spheroid

Chapters in this video

0:04

Introduction

0:27

Isolation and Culture of Human Keratinocytes from Neonatal Foreskin Tissue

2:37

Generating Skin Epidermosphere Cultures in Vitro

5:02

Epidermal Spheroid Re-Plating Assay

5:42

Characterization of Spheroid-Derived (SD) Sub-Populations by FACS

8:06

Results: Characterization of Epidermal Spheroid Formation and Spheroid-Derived NHKs

9:30

Conclusion

Related Videos

article

15:40

Forward Genetic Approach to Uncover Stress Resistance Genes in Mice — A High-throughput Screen in ES Cells

8.1K Views

article

07:33

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media

7.8K Views

article

08:05

Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research

10.1K Views

article

06:51

Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

20.7K Views

article

12:21

Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol

10.5K Views

article

07:18

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening

6.4K Views

article

09:14

Evaluation of Injury-induced Senescence and In Vivo Reprogramming in the Skeletal Muscle

9.4K Views

article

08:42

Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution

12.9K Views

article

06:49

A Semi-high-throughput Imaging Method and Data Visualization Toolkit to Analyze C. elegans Embryonic Development

6.6K Views

article

07:57

Generation and Culture of Lingual Organoids Derived from Adult Mouse Taste Stem Cells

4.1K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved