Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity

3.6K Views

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10:03 min

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January 30th, 2021

DOI :

10.3791/62182-v

January 30th, 2021

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Yvon Woappi¹^,², Geraldine Ezeka³, Justin Vercellino⁴, Sean M. Bloos⁵, Kim E. Creek⁶, Lucia Pirisi¹

¹Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, ²Department of Dermatology, Brigham and Women's Hospital, Harvard Medical School, ³Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine Baltimore, ⁴Department of Pathology, Albert Einstein College of Medicine, ⁵Department of Pediatrics, Vanderbilt University Medical Center, ⁶Department of Drug Discovery and Biomedical Sciences, College of Pharmacy, University of South Carolina

Summary

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Epidermal Spheroid Culture

Keratinocyte Stem Cell Plasticity

Soybean Trypsin Inhibitor

Chapters in this video

0:04

Introduction

0:27

Isolation and Culture of Human Keratinocytes from Neonatal Foreskin Tissue

2:37

Generating Skin Epidermosphere Cultures in Vitro

5:02

Epidermal Spheroid Re-Plating Assay

5:42

Characterization of Spheroid-Derived (SD) Sub-Populations by FACS

8:06

Results: Characterization of Epidermal Spheroid Formation and Spheroid-Derived NHKs

9:30

Conclusion

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