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Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution

DOI :

10.3791/57406-v

March 29th, 2018

March 29th, 2018

12,594 Views

1Laboratory of Stem Cell and Neuro-Vascular Biology, Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, 2Earle A. Chiles Research Institute, Robert W. Franz Cancer Center, Providence Portland Medical Center

Here, we describe a high resolution whole-mount imaging method in the entire adult mouse ear skin, which enables us to visualize branching morphogenesis and patterning of peripheral nerves and blood vessels, as well as immune cell distribution.

Tags

Whole mount Confocal Microscopy

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