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Temperature-Controlled Assembly and Characterization of a Droplet Interface Bilayer

DOI :

10.3791/62362-v

April 19th, 2021

April 19th, 2021

3,261 Views

1Department of Mechanical, Aerospace and Biomedical Engineering, University of Tennessee

This protocol details the use of a feedback temperature-controlled heating system to promote lipid monolayer assembly and droplet interface bilayer formation for lipids with elevated melting temperatures, and capacitance measurements to characterize temperature-driven changes in the membrane.

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