Forming a Uni-Lipid Supported Lipid Bilayer Using QCM-D
7:29
Forming a Suspended Lipid Bilayer
8:05
Developing Multi-Lipid Cell Mimicking Vesicles and Bilayers
8:48
Molecule Interaction Studies with Uni-Lipid and Multi-Lipid Supported Bilayers
9:29
Molecule Interaction Studies with Uni-Lipid and Multi-Lipid Suspended Bilayers
10:15
Results: Development of Uni- and Multi-Lipid Cell Mimicking Vesicles and Bilayers
11:51
Conclusion
Transcript
The construction of lipid bilayer membranes described in this protocol can be used to obtain crucial information about membrane interactions with pharmaceutical agents, environmental toxicants, and even biological compounds. These techniques can b
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This protocol describes the formation of cell mimicking uni-lipid and multi-lipid vesicles, supported lipid bilayers, and suspended lipid bilayers. These in vitro models can be adapted to incorporate a variety of lipid types and can be used to investigate various molecule and macromolecule interactions.