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In Situ Exploration of Murine Megakaryopoiesis using Transmission Electron Microscopy

DOI :

10.3791/62494-v

September 8th, 2021

September 8th, 2021

2,332 Views

1Université de Strasbourg, INSERM, EFS Grand Est, BPPS UMR-S 1255, FMTS

Here, we present a protocol to analyze ultrastructure of the megakaryocytes in situ using transmission electron microscopy (TEM). Murine bone marrows are collected, fixed, embedded in epoxy resin and cut in ultrathin sections. After contrast staining, the bone marrow is observed under a TEM microscope at 120 kV.

Tags

Megakaryopoiesis

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