In situ Imaging of the Mouse Thymus Using 2-Photon Microscopy

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15:00 min

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January 25th, 2008

DOI :

January 25th, 2008

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Ena Ladi¹, Paul Herzmark¹, Ellen Robey¹

¹Department of Molecular and Cell Biology, University of California, Berkeley

Summary

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In Situ Imaging

2 photon Microscopy

Thymocyte Development

Migration Of Thymocytes

Neonatal Chimeras

Hematopoietic Chimeras

Bone Marrow Injection

Thymus Dissection

Ex Vivo Imaging

DMEM Without Phenol Red

Diverse T Cell Repertoire

Chapters in this video

0:00

Title

0:41

Introduction

1:36

Adoptive Transfer

2:51

Dissection of Thymus

9:01

Preparing Thymus for Imaging

10:23

2-photon Imaging

12:05

Example of 2-photon Imaging Results

13:57

Conclusion

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