The objective of this procedure is to assess the microtubule organization and dynamics in vivo. Neurons are polarized cells with distinct axonal, dendritic, and synaptic compartments, maintained by their underlying cytoskeleton. Microtubules form
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A protocol for imaging the dynamic microtubules in vivo using fluorescently labeled End binding protein has been presented. We described the methods to label, image, and analyze the dynamic microtubules in the Posterior lateral microtubule (PLM) neuron of C. elegans.