Generation and Isolation of Extracellular Vesicles (EVs) Derived from Tumor Cells After Chemotherapy Exposure
2:59
Immunization of C57BL/6 Mice with Extracellular Vesicles (EVs) and Flow Cytometry Analysis of Splenic T Cells
6:01
Results: The Assessment of the Immunogenicity of Tumor-Derived Extracellular Vesicles (EVs)
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Conclusion
Transcript
This video shows how to assess the in vivo immunogenicity of tumor-derived extracellular vesicles, or EVs, using flow cytometry. The screening method described herein is relatively easy to perform and can be adapted to various settings. In cancer,
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This manuscript describes how to assess in vivo immunogenicity of tumor cell-derived extracellular vesicles (EVs) using flow cytometry. EVs derived from tumors undergoing treatment-induced immunogenic cell death seem particularly relevant in tumor immunosurveillance. This protocol exemplifies the assessment of oxaliplatin-induced immunostimulatory tumor EVs but can be adapted to various settings.