Generation and Functional Verification of Hypoxia-Sensitive Chimeric Antigen Receptor-T Cells

678 Views

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09:12 min

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June 14th, 2024

DOI :

10.3791/66697-v

June 14th, 2024

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Ying Xue*¹, Yunyu Mao*², Qibin Liao³, Chen Zhao⁴, Xiaoyan Zhang¹^,²^,⁴, Jianqing Xu¹^,²^,⁴

¹Institutes of Biomedical Sciences, Fudan University, ²Clinical Center of Biotherapy at Zhongshan Hospital, Fudan University, ³Department of Oncology and Bio-therapeutic Center, Shenzhen Third People's Hospital, Southern University of Science and Technology, ⁴Shanghai Public Health Clinical Center, Fudan University

Transcript

Summary

Explore More Videos

Hypoxia sensitive CAR T cells

Cancer Immunotherapy

CAR T cell Therapy

Tumor Microenvironment

Tumor Selectivity

Chimeric Antigen Receptor

Hypoxia conditioned CAR

Safety Profile

Efficacy Enhancement

Functional Verification

Oxygen dependent Degradation Domain

Cancer Treatment Protocol

Cytotoxicity Analysis

Mobile Incubator Chamber

Chapters in this video

0:00

Introduction

1:43

Development of Hypoxia‐Responsive CAR‐T Cells via Lentiviral Transduction

4:32

Flow Cytometric Assessment of Oxygen‐Dependent CAR Expression in CAR‐T cells

7:14

In Vitro Assessment of Cytotoxicity Mediated by Hypoxia‐Sensitive CAR‐T Cells

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