JoVE Logo
Faculty Resource Center

Sign In

A Spheroid Killing Assay by CAR T Cells

DOI :

10.3791/58785-v

December 12th, 2018

December 12th, 2018

14,546 Views

1Department of Cellular Therapy, Department of Oncology, Oslo University Hospital-Radiumhospitalet

This protocol is designed to assess immunotherapeutic redirected T-cell (CAR T-cell) cytotoxicity against 3D structured cancerous cells (spheroids) in real time.

Tags

Spheroid Killing Assay

-- Views

Related Videos

article

Preparation and Metabolic Assay of 3-dimensional Spheroid Co-cultures of Pancreatic Cancer Cells and Fibroblasts

article

A Comprehensive Procedure to Evaluate the In Vitro Performance of the Putative Hemangioblastoma Neovascularization Using the Spheroid Sprouting Assay

article

A 3D Organotypic Melanoma Spheroid Skin Model

article

A Syngeneic Mouse B-Cell Lymphoma Model for Pre-Clinical Evaluation of CD19 CAR T Cells

article

Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells

article

Fully Human Tumor-based Matrix in Three-dimensional Spheroid Invasion Assay

article

Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells

article

Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells

article

Manufacturing Chimeric Antigen Receptor (CAR) T Cells for Adoptive Immunotherapy

article

Isolation of Stem-like Cells from 3-Dimensional Spheroid Cultures

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved