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Measuring Mitochondrial Substrate Flux in Recombinant Perfringolysin O-Permeabilized Cells

DOI :

10.3791/62902-v

August 13th, 2021

August 13th, 2021

2,244 Views

1Department of Physiology, Faculty of Medicine in Hradec Králové, Charles University, 2Department of Pathophysiology, Third Faculty of Medicine, Charles University, 3Department of Internal Medicine, University Hospital Kralovske Vinohrady

In this work, we describe a modified protocol to test mitochondrial respiratory substrate flux using recombinant perfringolysin O in combination with microplate-based respirometry. With this protocol, we show how metformin affects mitochondrial respiration of two different tumor cell lines.

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Keywords Mitochondrial Substrate Flux

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