JoVE Logo
Faculty Resource Center

Sign In

Exploring Mitochondrial Energy Metabolism of Single 3D Microtissue Spheroids Using Extracellular Flux Analysis

DOI :

10.3791/63346-v

February 3rd, 2022

February 3rd, 2022

2,541 Views

1School of Biosciences, University of Birmingham, 2Nanomedicine, Drug Delivery & Nanotoxicology Lab, School of Pharmacy, University of Birmingham, 3Mitochondrial Profiling Centre, University of Birmingham

These protocols will help users probe mitochondrial energy metabolism in 3D cancer cell-line-derived spheroids using Seahorse extracellular flux analysis.

-- Views

Related Videos

article

Simple and Rapid Method to Obtain High-quality Tumor DNA from Clinical-pathological Specimens Using Touch Imprint Cytology

article

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

article

Studying Normal Tissue Radiation Effects using Extracellular Matrix Hydrogels

article

Flow Cytometric Analysis of Mitochondrial Reactive Oxygen Species in Murine Hematopoietic Stem and Progenitor Cells and MLL-AF9 Driven Leukemia

article

Physiologic Patient Derived 3D Spheroids for Anti-neoplastic Drug Screening to Target Cancer Stem Cells

article

Establishment and Characterization of Small Bowel Neuroendocrine Tumor Spheroids

article

Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells

article

Analytical Determination of Mitochondrial Function of Excised Solid Tumor Homogenates

article

Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy

article

Freeze-Fracture Electron Microscopy for Extracellular Vesicle Analysis

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved