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DOI :
10.3791/63068-v
•
10:45 min
October 14th, 2021
Chapters
0:05
Introduction
1:11
Embryo Extraction
1:44
Ex Utero and In Utero Electroporation
3:35
Brain Extraction and Embedding in Agarose
4:39
Organotypic Slice Culture
5:43
Image Acquisition and Data Analysis
8:29
Results: In Situ Axon Growth and Growth Cone Dynamics
9:56
Conclusion
このプロトコルは、 その場で 軸索の成長および成長円錐ダイナミクスを研究するための簡単で堅牢な方法を実証する。 これは、生体外で 生理学的に関連する急性脳スライスを調製する方法を説明し、ユーザーフレンドリーな分析パイプラインを提供します。
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