JoVE Logo
Faculty Resource Center

Sign In

Collection, Expansion, and Differentiation of Primary Human Nasal Epithelial Cell Models for Quantification of Cilia Beat Frequency

DOI :

10.3791/63090-v

November 10th, 2021

November 10th, 2021

3,359 Views

1School of Women's and Children's Health, Faculty of Medicine and Health, University of New South Wales, 2Molecular and Integrative Cystic Fibrosis Research Centre (miCF RC), Faculty of Medicine and Health, University of New South Wales, 3Department of Respiratory Medicine, Sydney Children's Hospital, 4Department of Pathology, School of Medical Sciences, University of New South Wales Australia, 5Katharina Gaus Light Microscopy Facility, Mark Wainwright Analytical Centre, University of New South Wales

This protocol describes nasal epithelial cell collection, expansion, and differentiation to organotypic airway epithelial cell models and quantification of cilia beat frequency via live-cell imaging and custom-built scripts.

Tags

Primary Human Nasal Epithelial Cell Models

-- Views

Related Videos

article

Collection Protocol for Human Pancreas

article

Processing of Primary Brain Tumor Tissue for Stem Cell Assays and Flow Sorting

article

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

article

Time-lapse Imaging of Primary Preneoplastic Mammary Epithelial Cells Derived from Genetically Engineered Mouse Models of Breast Cancer

article

Primary Culture of Human Vestibular Schwannomas

article

Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications

article

High-frequency Ultrasound Imaging of Mouse Cervical Lymph Nodes

article

Isolation and Culture Expansion of Tumor-specific Endothelial Cells

article

Absorption of Nasal and Bronchial Fluids: Precision Sampling of the Human Respiratory Mucosa and Laboratory Processing of Samples

article

Generation of Human Nasal Epithelial Cell Spheroids for Individualized Cystic Fibrosis Transmembrane Conductance Regulator Study

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved